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Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.v.

Nenad Gajovic-Eichelmann

Rudolf Ehwald

Helge Adleff

Frank Bier

Max Ehwald

Burkhard Micheel

Julia Ettlinger

Gudrun Scharte

Jörg Schenk

A method for performing homogeneous immunoassay formats with electrochemical detection in solution includes combining two different conjugates as reagents with a sample or a sample/buffer mixture, one conjugate including a redox marker and an analyte molecule and the second conjugate including an anti-redox marker antibody or a specifically binding fragment thereof and a molecule specifically binding the analyte.

Method for electrochemical detection of binding reactions

The invention relates to an assay method and a fluid microsystem for carrying out said assay method, in particular, for carrying out miniaturized affinity tests in a micro-array format. According to said invention, a liquid phase comprising at least one type of detectable high-molecular weight soluble substance, for example a labeled reaction partner or product of an affinity reaction is displaced by the hydraulic effect of a high-molecular weight osmotic agent on an ultrafiltration membrane towards a miniaturized measuring chamber defined thereby. The fraction of the detectable high-molecular weight substance(s) is concentrated in the measuring chamber, while the dissolved low-molecular weight components are removed with the solvent through the membrane pores, thereby making it possible to attain a high detection sensitivity.

Assay by osmotically induced separation and concentration of high-molecular detectable substances and a fluid microsystem for carrying out said assay

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Berlin, Germany

Nenad Gajovic-Eichelmann