Dsm IP Assets B.v.

Verenium Corporation

Bunge Oils, Inc.

Diversa Corporation

Bunge Global Innovation, LLC

Other

Genomatica, Inc.

Basf Enzymes LLC

Nelson Robert Barton

David B Weiner

Jonathan D Lyon

Tim S Hitchman

Mark A Wall

Christopher L G Dayton

Eileen O'Donoghue

Katie Kline

Kevin A Gray

David E Lam

Jay Milton Short

Dan Eric Robertson

David Paul Weiner

James B Garrett

Mark Joseph Burk

Gerhard Johann Frey

Eric J Mathur

Toby Howard Richardson

Walter Callen

Janne Samuli Kerovuo

Malgorzata Slupska

Geoff Hazlewood

Analia Bueno

Xuqiu Tan

Robert Christopher Brown

Keith A Kretz

Carl A Miller

Giselle G Janssen

Joel Kreps

Priti Pharkya

Connie Jo Hansen

Michelle H Cayouette

Paul S Zorner

Justin Stege

Nahla M Aboushadi

Jingyi Li

Joslin M Cuenca

Svetlana Gramatikova

Christopher L Dayton

Mark L Miller

Timothy Hitchman

Joslin G Cuenca

Eileen O'donoghue

Katie A Kline

Amit Vasavada

Jincai Li

Blake G Sturgis

Roderick James Fielding

Adrian Badillo

Wilhelmus P Van Hoek

Charles Isaac

Flavio Da Silva Galhardo

Joslin Cuenca

Mark Dycaico

Joseph R Warner

Giselle Janssen

Amy McClure

May Sun

W Michael Lafferty

Kevin Chow

William Baum

Carl Miller

Flavio Da Galhardo

Joel A Kreps

Roderick Fielding

Robert C Brown

Matgorzata Slupska

Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

Methanol dehydrogenase fusion proteins

In alternative embodiments, the invention provides phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes, nucleic acids encoding them, antibodies that bind specifically to them, and methods for making and using them. Industrial methods and products comprising use of these phospholipases are also provided. In certain embodiments, provided herein are methods for hydration of non hydratable phospholipids (NHPs) within a lipid matrix. The methods enable migration of NHPs to an oil-water interface thereby allowing the NHPs to be reacted and/or removed from the lipids. In certain embodiments, provided is a method for removing NHPs, hydratable phospholipids, and lecithins from vegetable oils to produce a degummed oil or fat product that can be used for food production and/or non-food applications. In certain embodiments, provided herein are methods for hydration of NHPs followed by enzymatic treatment and removal of various phospholipids and lecithins. The methods provided herein can be practiced on either crude or water-degummed oils.

Phospholipases, nucleic acids encoding them and methods for making and using them

Oil degumming methods

The invention provides novel methods for making or modifying oils, e.g., plant animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof.

Compositions and methods for making and modifying oils

Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

Hydrolases, nucleic acids encoding them and methods for making and using them

The invention provides the invention provides compositions and methods for the enzymatic treatment ('bleaching' or 'de-colorizing') of chlorophyll-comprising compositions, e.g., algae preparations, chlorophyll-containing or chlorophyll-contaminated feeds, foods or oils, for example, vegetable oils, including oils processed from oilseeds, such as canola (rapeseed) oil or soybean oil, or oil fruits, such as palm oil. In one aspect, the invention provides methods using a chlorophyllase enzyme for the enzymatic hydrolysis of chlorophyll in an algae, an animal (e.g., a fish) or plant preparation, a food or an oil. In one aspect, the chlorophyllase is immobilized onto a silica. The invention also provides compositions of manufacture and detergents.

Methods for enzymatic decolorization of chlorophyll

The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.

The invention provides novel methods for making or modifying oils, e.g., plant, animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, low linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof.

Hydrolases, nucleic acids encoding them and methods for biocatalytic synthesis of structured lipids

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San Diego, CA, United States of America

Nelson Robert Barton