Ventana Medical Systems, Inc.

Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Nelson Alexander

Stacey Stanislaw

Larry E Morrison

Jerome W Kosmeder, Ii

William Kenneth Day

Thomas M Grogan

Aoune Barhoumi

Lisa L Gallegos

Mark Lefever

Anne Pedata

Jigar Patel

Jan Berka

Sedide Ozturk

Esteban Roberts

Rui Chen

Alisa Tubbs

Seoyoung Kim

Toumy Guettouche

Daniel Burgess

Hiro Nitta

Florian Rubelt

Maeve E O'Huallachain

Heidi J Rosenbaum

Samantha Rajkovich

Melinda Day

Eric Walk

Katherine Leith

Leigh A Henricksen

Jerome W Kosmeder

Heidi Rosenbaum

William Day

Disclosed herein is a method of analyzing flow cytometry data for cells derived from homogenized whole tumor samples.

Size-based gating to analyze flow cytometry data

The disclosure generally relates to the preparation of representative samples from clinical samples, e.g., tumors (whole or in part), lymph nodes, metastases, cysts, polyps, or a combination or portion thereof, using mechanical and/or biochemical dissociation methods to homogenize intact samples or large portions thereof. The resulting homogenate provides the ability to obtain a correct representative sample despite spatial heterogeneity within the sample, increasing detection likelihood of low prevalence subclones, and is suitable for use in various diagnostic assays as well as the production of therapeutics, especially 'personalized' anti-tumor vaccines or immune cell based therapies.

Representative diagnostics

Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

Signaling conjugates and methods of use

The present disclosure provides a method of separating cellular particles from a tissue sample and then sorting the cellular particles into two or more cellular particle populations.

Size-based separation of dissociated fixed tissues

In one aspect of the present disclosure is a targeted sequencing workflow where an input sample comprising a sufficient quantity of genomic material is provided such minimal or no amplification cycles are utilized prior to sequencing.

Deep sequencing profiling of tumors

The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

System for identification of antigens recognized by T cell receptors expressed on tumor infiltrating lymphocytes

Growing community of inventors

Marana, AZ, United States of America

Nelson Alexander