Ajinomoto Co., Ltd.

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Nelli Isaakovna Zhdanova

Mikhail Markovich Gusyatiner

Vitaly Arkadievich Livshits

Nikolai Kazimirovich Yankovsky

Evgeny Moiseevich Khurges

Jury I Kozlov

Albert Fedorovich Sholin

Alexandr Konstantinovich Sokolov

Tatyana Alexandrovna Bachina

Raisa Alexandrovna Arsatiants

Tamara Mikhailovna Pozdnyakova

Vladimir Georgievich Debabov

Alla Valentinovna Belareva

Jury Dmitrievich Tsygankov

Tatyana Grigorievna Plotnikova

Vladimir G Debabov

Andrei Jurievich Chistoserdov

Irina Olegovna Shakalis

Irina Clegovna Shakalis

Ljudmila F Kozyreva

Tatyana Viktorovna Leonova

Sergei Vladimirovich Mashko

Rustem Saidovich Shakulov

Andrei J Chistoserdov

Jury Ivancvich Kozlov

Oleg Alexandrovich Rodionov

Alla Lvovna Lapidus

Lev Mikhailovich Evstjugov-Babaev

Evgeny Mciseevich Khurges

Roza Mitrofanovna Balitskaya

Andrei Jurievich Christoserdov

Natalya Nikolaevna Kuznetsova

Boris A Rebentish

Vera N Moshentseva

Vytalyi Arkadievich Lifshits

Viktor P Antipov

Tatyana Borisovna Kasatkina

Evgenyi Moiseevich Khurges

Uri Ivanovich Kozlov

Mikhail N Rozinov

Michail Markovich Gusyatiner

Alexander Konstantinovich Sokolov

Andrei Yurevich Christoserdov

Uri Dmitrievich Tsigankov

Tatiana Alexandrovna Bachina

Sergi Vladimirovich Mashko

Oksana Fedorovna Gavrilova

A bacterial strain ofis described which produces L-threonine, and is obtained by a process comprising transduction by bacteriophage P1 which bears a transposon which inactivates threonine dehydrogenase activity, and isolation of a transductant lacking threonine dehydrogenase activity.

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The present invention provides a method for deriving an strain which produces L-theronine from strain VNIIgenetika 472T23 by transducing with bacteriophage P1 which bears a transposon.

Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine

strains derived form,strain VNIIgenetika 472t23 and obtained by a process involving transduction by bacteriophage P1 which bears a transposon which inactivates threonine dehydrogenase activity and isolation of a transductant lacking threonine dehydrogenase activity are useful for producing L-threonine.

Escherichia coli strain and method for producing L-threonine

A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antiobiotics Research Institute under Reg. No. 1867.

Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of

The present invention provides a novel microbial strain and a method for effectively producing L-threonine. The novel strain can grow on a medium containing molasses, a much cheaper raw material than sucrose. The exemplary novel strain E. coli BKIIM B-5318 bears the plasmid pPRT614, which has threonine biosynthesis genes (thr A, B and C), the expression of which is regulated by a lambda-phage PR promoter and temperature-sensitive C1 repressor. The present microorganism is prototrophic with regard to isoleucine. The strain E. coli BKIIM B-5318 produces more than 70 g/l of L-threonine when cultured at 38-41.degree. C. for 32 hours in a medium containing molasses.

L-threonine-producing microbacteria and a method for the production of

A bacterial strain of Escherichia coli BKIIM B-3996, a producer of L-threonine, containing a recombinant plasmid pVIC40 and deposited on Nov. 19, 1987 in the collection of microorganism cultures at the USSR Antibiotics Research Institute under Reg. No. 1867.

A bacterial strain of Escherichia coli VNII genetika 472T23, a producer of L-threonine, containing the plasmid PYN7 and a plasmid-free variant of E. coli; strain VNII genetika 472T23, deposited at the American Type Culture Collection under Accesion No. ATCC 98081 and 98082, respectively.

Bacterial strain of Escherichia coli VNII genetika 472T23 as the

Bacterial strain of escherichia coli BKIIM B-3996 as the producer of

The process for producing L-threonine consists in that there is cultivated a producer of L-threonine in the capacity of which is used the Escherichia coli strain VNIIgenetika M-1 deposited in the Central museum of commercial microorganisms under the All-Union Research Institute for Genetics and Selection of Commercial Microorganisms at a registration No. IIMIIB-1856. The above strain has been selected on the basis of natural variability of the Escherichia coli strain VNIIgenetika VL 334/p YN7), obtained by virtue of the genetic engineering techniques through increasing the dose of mutant genes capable of a higher rate of L-threonine production, by introducing a multicopy hydrid plasmid carrying said genes, into a mutant recipient strain. The abovesaid producer is cultivated on a nutrient medium, containing sources of carbon, nitrogen, and some mineral salts in the presence of an antibiotic penicillin, the resultant biomass being then separated from the culture fluid, whereupon the end product is isolated.

Process for producing L-threonine

A method for constructing strains which produce aminoacids comprising combining of a DNA chromosome fragment of a donor microorganism containing genes controlling the synthesis of a selected aminoacid and having a mutation destroying the negative regulation of the synthesis of this aminoacid with a vector DNA molecule to form a hybrid DNA molecule. Use is made of a vector DNA molecule capable of providing amplification of the hybrid DNA molecule. The resulting hybrid DNA molecule is used for transforming cells of the recipient strain having the mutation blocking the synthesis of the selected aminoacid in this strain and the mutation partly blocking the related step of metabolism of this aminoacid to yield the strain capable of increased productivity of the selected aminoacid.

Method for preparing strains which produce aminoacids

A method of producing L-isoleucine which comprises culturing the strain of Brevibacterium flavum VNII Genetika 10-89 deposited under the number CMIM B-1507 in a nutrient medium containing sources of nitrogen, carbon, mineral salts and vitamins and under aerobic conditions. Said strain makes it possible to produce L-isoleucine with a yield of 17 g/l and more.

Method of producing L-isoleucine using Brevibacterium flavum

The method of preparing L-glutamic acid and its sodium salt by growing, on a nutrient medium containing sources of nitrogen, carbon and mineral salts a strain producent of L-glutamic acid, Micrococcus glutamicus 'VNIIgenetika' 3144, obtained from the strain Micrococcus glutamicus 'VNIIGenetika' 490 by multi-step selection with mutagenic factors-diethyl sulphate and ultra-violet radiation-- the biotin concentration in the liquid mineral medium preferably being such as to ensure the maximum yield of L-glutamic acid.

Method of preparing l-glutamic acid and its sodium salt

Growing community of inventors

Moscow, Russia

Nelli Isaakovna Zhdanova