3m Innovative Properties Company

Neogen Food Safety US Holdco Corporation

Neil Percy

Gregory W Sitton

James E Aysta

Mark B Driscoll

Catherine Mary Ramsay

Rachel E Evans

Kurt J Halverson

Manjiri T Kshirsagar

Jesse D Miller

Kenneth R Davenport

Jerald K Rasmussen

Jimmie Rae Baran, Jr

Kannan Seshadri

Clinton P Waller, Jr

Douglas Eugene Weiss

Steven P Swanson

Jason W Bjork

Stephanie J Moeller

Tonya D Bonilla

Jeffrey A Lucas

Cheryl A Bartholomew

Christina A Barnes

Clinton P Waller

Primers and methods of amplifying DNA from shiga toxins stx1 and stx2 designed for use in a multiplex method and useful in detecting shiga-toxin producing(STEC). The primers being especially designed for use in Loop-mediated Isothermal Amplification (LAMP).

Loop-mediated isothermal amplification primers for Shiga toxin-producing(STEC) detection

An apparatus and kit for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity that is not substantially defined by space between a plurality of fibers. The sampling device comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.

Apparatus for detecting ATP in a liquid sample

The present disclosure provides a nucleic acid amplification method. The method comprises forming an enrichment culture by contacting a sample with a nutrient medium having a formulation that does not include a phosphate buffer component; holding the enrichment culture for a period of time at a temperature that facilitates growth of a target microorganism; after holding the enrichment culture, forming an aqueous composition by mixing a first volume of the enrichment culture with a second volume of a lysis buffer; contacting the aqueous composition with an effective amount of a water-insoluble material that sequesters a substance that interferes with a polymerase-mediated nucleic acid amplification reaction; subjecting the aqueous composition to a thermal lysis process; and, after subjecting the aqueous composition to the thermal lysis process, subjecting a portion of the aqueous composition to a nucleic acid amplification process. A composition for a lysis buffer is also disclosed.

Process for cell lysis and nucleic acid amplification

Apparatuses and kits for the detection of ATP in a liquid sample are provided. The apparatuses and kits comprise a liquid reagent composition comprising luciferin and a sampling device having a sampling portion and a handling portion. The sampling portion comprises a fibrous or a foam material and is adapted to acquire and releasably retain a predetermined volume of a liquid sample or a sample of residue from a surface. The sampling device comprises a dry coating or a liquid coating, the coating including an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. Methods of use of the apparatus or kit are also provided.

Apparatus and methods for detecting ATP in a liquid sample

An apparatus () and kit () for the detection of ATP in a liquid sample is provided. The apparatus and kit comprise a liquid reagent composition comprising luciferin and a sampling device having () a sampling portion () and a handling portion (). The sampling portion () is adapted to acquire and releasably retain a predetermined volume of a liquid sample in one or more cavity () that is not substantially defined by space between a plurality of fibers. The sampling device () comprises a dry coating that includes an effective amount of a pH-adjusting reagent that, when contacted with a liquid reagent composition having a pH of about 6.8 or lower, changes the pH of the liquid reagent composition to 6.9 or higher. A method of use of the apparatus or kit is also provided.

A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target cell-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target cell-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target cell-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target microorganism are also provided.

Method of detecting a microorganism using chromatographic enrichment

A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes effective amounts of ferric iron, an organic iron-chelating reagent, and a non-ionic surfactant. Optionally, the composition includes polyvinylpyrrolidone. The composition has a pH of about 8.45 to 8.85. The organic iron-chelating reagent has a first affinity constant greater than or equal to 10with respect to ferric iron and a second affinity constant less than 10with respect to magnesium. The first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20&#xb0; C. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.

Composition for reducing inhibition of nucleic acid amplification

A device is provided. The device comprises a casing comprising an interior, a first opening, and a second opening; and a porous carrier comprising a sample-receiving zone and a target analyte-binding zone. The porous carrier defines a first fluid pathway that extends from the sample-receiving zone to the target analyte-binding zone. At least portion of the porous carrier is disposed in the interior of the casing. A second fluid pathway comprising a central axis extends through the casing from the first opening and the second opening, the second fluid pathway intersecting the porous carrier at the target analyte-binding zone. The central axis is oriented orthogonally with respect to the porous carrier. Methods of using the device to detect a target analyte are also provided.

Method of detecting an analyte using chromatographic enrichment

The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.

Sampling devices and methods for concentrating microorganisms

A method of detecting an analyte is provided. The method includes providing a sample, a containerwith a wall, and a catalyst for a luminescent reaction. The wall includes a colored portion. The method further comprises forming a reaction in the container and detecting the presence or absence of light emitted from the reaction mixture in the container. Detecting light emitted from the container can comprise detecting light passing through the colored portion. The colored portion can be detected visually and the color can be associated with the identity of an analyte-specific reagent disposed in the container. Kits comprising the container and a catalyst for a luminescent reaction are also provided.

Luminescence detection method

Tools () are provided for use in uncapping one or more of a plurality of linearly-oriented, spaced-apart tubes; each cap having a cap upper surface with a depression. The tools comprise a body () having first portion () for engagement by a user and a second portion () for engaging a plurality of caps. The second portion comprises a plurality of spaced-apart projections (). Each projection is configured for releasably engaging a cap. The projection may comprise two or more spaced-apart projection elements (). Optionally, the first portion is configured in a non-coplanar relationship with respect to the second portion. Methods of use are also provided.

Cap handling tools and methods of use

A microbial detection article and methods of using the same, the article comprising: a base member comprising a self-supporting water impervious substrate with first and second generally opposed major surfaces; a filter assembly defining a filter assembly aperture therein, and having a composite filter body mounted across the filter assembly aperture; wherein the composite filter body comprises: a microporous membrane, and a water-absorbent layer in fluid communication with the microporous membrane; and a cover sheet.

Growing community of inventors

Saint Paul, MN, United States of America

Neil Percy