Emd Millipore Corporation

Genetics Institute, Inc.

Comera Life Sciences, Inc.

Neil L Schauer

David S Soane

Robert P Mahoney

Edward R LaVallie

Martin Morrissey

Philip Wuthrich

Mark Moody

Jeffrey S Deetz

Daniel G Greene

Steven M Vicik

James R Mercer

Dwight Winters

Jenifer L Thomas

Catherine A Briasco

Edward R La Vallie

Disclosed herein are methods for improving a parameter of a protein-related process comprising providing a viscosity-reducing excipient compound selected from the group consisting of hindered amines, anionic aromatics, functionalized amino acids, oligopeptides, short-chain organic acids, and low molecular weight aliphatic polyacids, and adding a viscosity-reducing amount of the viscosity-reducing excipient compound to a carrier solution for the protein-related process, wherein the carrier solution contains a protein of interest, and carrier solutions comprising a liquid medium in which is dissolved a protein of interest, and a viscosity-reducing excipient, wherein the viscosity of the carrier solution has a lower viscosity that that of a control solution that is substantially similar to the carrier solution except for the presence of the viscosity-reducing excipient.

Excipient compounds for protein processing

A disposable flow path having a manifold system that provides pressure resistance for the disposable device. The disposable device is comprised of a first rigid plastic sheet and a second rigid plastic sheet. Each sheet has two major surfaces and a thickness between them. At least one sheet surface has flow channels formed in it. The flow channels extend away from a first major surface and beyond the normal plane of a second major surface of the sheet. The sheets are liquid tightly sealed to each other at their adjoining first major surfaces. When each sheet contains a flow channel, the flow channels are aligned and in register with each other. One or more fittings can be secured in the flow channel(s) at an edge of the two liquid tightly sealed sheets. Alternatively, tubing is placed in the channels before the sheets are secured to one another.

Rigid disposable flow path

Provided by the present invention are novel methods of protein recovery and purification methods and more specifically novel methods for the recovery and purification thioredoxin fusion proteins, especially of IL-11.

Protein recovery & purification methods

Growing community of inventors

Milford, MA, United States of America

Neil L Schauer