Endocellutions, Inc.

Cervos Medical LLC

Ecw Therapeutics, Inc.

Neil Francis Duffy

Andrew McGillicuddy

Jeffrey Raymond Chabot

Andy H Levine

John C Meade

Neil Tischler

Edward H Kislauskis

A system and associated method for concentrating and separating components of different densities from fluid containing cells using a centrifuge includes a container defining a cavity for receiving the fluid. The container has a top, a sidewall extending from the top, and a bottom disposed opposite the top and in sealing engagement with the sidewall. An insert is slidably disposed in the cavity of the container and defines a lumen through the insert. The lumen, which includes a hole and a funnel-shaped upper portion in fluid communication with the hole, forms an open fluid path between opposite ends of the insert. The insert has a density such that upon centrifugation a selected component of the fluid resides within the lumen. A container port is disposed in the top of the container to transfer the fluid into the container and to withdraw a fluid component other than the selected component from the container. The system includes a manifold that includes a manifold port, a vent to vent the container, and a connector to couple to the container port. A cannula is receivable in the manifold port and extendable through the container port into the container and into the lumen of the insert to withdraw the selected component from the lumen.

Cell concentration devices and methods including a syringe and a syringe holder

A system and associated method for concentrating and separating components of different densities from fluid containing cells using a centrifuge includes a syringe and a syringe holder, the syringe having a proximal top with a luer port, a sidewall extending from the top forming a syringe tube, and a plunger slidably disposed inside the syringe tube, the plunger forming a sealing engagement with the sidewall, the syringe holder defining a cavity for receiving the syringe, wherein a distal end of the syringe tube is at least partially closed by the syringe holder after the plunger is placed inside the syringe tube. The method includes receiving a fluid containing cells in the syringe; placing the syringe and syringe holder into a centrifuge;

A bone marrow aspiration device and associated method includes an introducer needle and an aspiration needle. The introducer needle includes an introducer cannula and a removable introducer stylet that includes a sharp tip to penetrate bone. The aspiration needle includes an optionally flexible aspiration cannula and a blunt stylet. The aspiration needle is receivable in the introducer cannula when the introducer stylet is removed from the introducer needle. The aspiration cannula forms a channel for aspirating bone marrow when the stylet is removed. The aspiration device also includes a locking mechanism to lock the aspiration cannula to the introducer cannula when the aspiration cannula is received in the introducer cannula, whereby the aspiration cannula can be advanced distally relative to the introducer cannula in a controlled manner, and whereby pulling the aspiration cannula proximally causes the introducer cannula to move proximally with the aspiration cannula.

Apparatus and methods for aspirating tissue

Cell concentration devices and methods that include an insert defining a lumen and a cannula assembly

A system for separating components defining a cavity for receiving the fluid. The system further includes a rigid insert slidably disposed in the cavity, the insert including a funnel-shaped upper portion and a hole therethrough. The insert has a density such that upon centrifugation a selected component of the fluid resides within the upper portion of the insert. A bone marrow aspiration device includes an introducer needle assembly and an aspiration needle assembly. The introducer needle assembly includes an introducer cannula and a removable trocar. The aspiration needle assembly includes a flexible aspiration cannula and a flexible stylet. The length of the aspiration cannula is substantially greater than the length of the introducer cannula. The aspiration needle assembly is receivable in the introducer cannula when the trocar is removed from the introducer needle assembly. The aspiration cannula forms a channel for aspirating bone marrow when the stylet is removed.

Apparatus and methods for aspirating and separating components of different densities from a physiological fluid containing cells

The anatomic and functional arrangement of the gastrointestinal tract suggests an important function of this organ is its ability to regulate the trafficking of metabolites as well as control the equilibrium between tolerance and immunity through gut-associated lymphoid tissue, the neuroendocrine network, and the intestinal epithelial barrier. Combining nucleated cells from various tissues and introducing them directly into the small intestine will have a positive effect on diabetes.

Adult and neonatal stem cell therapy to treat diabetes through the repair of the gastrointestinal tract

A system for separating components defining a cavity for receiving the fluid. The system further includes a rigid insert sSidably disposed in the cavity, the insert including a funnel-shaped upper portion and a hole therethrough. The insert has a density such that upon centrifugation a selected component of the fluid resides within the upper portion of the insert. A bone marrow aspiration device includes an introducer needle assembly and an aspiration needle assembly. The introducer needle assembly includes an introducer cannula and a removable trocar. The aspiration needle assembly includes a flexible aspiration cannula and a flexible stylet. The length of the aspiration cannula is substantially greater than the length of the introducer cannula. The aspiration needle assembly is receivable in the introducer cannula when the trocar is removed from the introducer needle assembly. The aspiration cannula forms a channel for aspirating bone marrow when the stylet is removed.

Disclosed herein are apparatus and methods for isolating a fraction of interest from a physiological fluid sample. A sample holder for isolating a fraction of interest from a physiological fluid sample includes a flexible compartment and a rigid exoskeleton that supports the flexible compartment. The flexible compartment may have at least one reservoir with a height to volume ratio of about 0.1 cm/mL to about 5 cm/mL. An automated device for extracting a fraction of interest from the sample includes a sample holder with a flexible compartment supported by a rigid exoskeleton, a support for the sample holder connected to one or more fluid extraction devices, and a motor for moving the extraction device relative to the sample holder. The automated device may include an optical sensor and may include a clamp for clamping the flexible compartment.

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Brighton, MA, United States of America

Neil Francis Duffy