Arizona State University

Other

Arizona Board of Regents, a Body Corporate

Neal W T Woodbury

Stephen Albert Johnston

Hao Yan

Phillip Stafford

Jinglin Fu

Zhao Zhao

Zhang-Gong Zhao

Alexander A Green

Mark A Hayes

Thomas J Taylor

John C Chaput

Matthew Paul Greving

Nicholas Stephanopoulos

Benjamin P Bowen

Nidhi Gupta

Christopher W Diehnelt

Matthew Gilliam

Allan Scruggs

Zhan Gong Zhao

Kirstie Swingle

Martin Thompson

John Rajasekaran

Karl Booksh

Pierre Herckes

The present disclosure describes a nanoparticle comprising a three dimensional DNA nanocage and a payload biological macromolecule, and methods of assembly thereof.

Nanocaged enzymes with enhanced catalytic activity and increased stability

Systems, devices and methods for predicting binding on an array such as a peptide array. Certain methods utilize a peptide array having a plurality of peptides with one or more defined parameters and contacting the peptide array with a training sample containing one or more molecules of interest. Interactions between the plurality of peptides and the one or more molecules of interest are processed according to a data fitting model, which model is then is applied to interactions between the plurality of peptides and a test sample to predict binding associated with the one or more molecules of interest.

Prediction of binding from binding data in peptide and other arrays

Methods and systems, including those employing machine learning, utilizing one or more algorithms for relating the structure of a molecule in a library to its function are described. Embodiments described herein relate structure to function by considering the covalent structure of the molecule, the components of that structure that are common to many molecules in the library, and the properties of those components as they relate to the function in question. Applications include, for example, enhancement and amplification of the diagnostic and prognostic signals provided by peptide arrays for use in analyzing the profile of antibodies in the blood produced in response to a disease, condition or treatment.

Computational analysis to predict molecular recognition space of monoclonal antibodies through random-sequence peptide arrays

Described herein are in situ synthesized arrays and methods of making the them, wherein array signal sensitivity and robustness is enhanced by carrying out conditioning steps and/or generating linkers during synthesis. An array comprises a surface with a collection of features, wherein the features comprise molecules or polymers attached to the surface. In certain embodiments of the invention, carrying out conditioning steps during array synthesis can yield arrays with improved signal. In other embodiments, linkers are synthesized on the array surface prior to synthesis of functional molecules, wherein increasing linker length can correspond to an improvement in the signal generated by the array.

Conditioned surfaces for in situ molecular array synthesis

Integrated diagnostic devices comprising peptide-DNA conjugates for analyte detection, an embedded biomolecular computing system for sample analysis, and a layered device architecture are provided herein. In particular, provided herein are devices comprising a layered architecture that enables diagnostic reagents, sample components, and reaction products to flow through the system with minimal user intervention.

Integrated diagnostic devices having embedded biomolecular computing systems and uses thereof

The present application provides arrays for use in immunosignaturing and quality control of such arrays. Also disclosed are peptide arrays and uses thereof for diagnostics, therapeutics and research.

Peptide array quality control

Described herein are in situ synthesized arrays and methods of making them, wherein array signal sensitivity and robustness is enhanced by carrying out conditioning steps and/or generating linkers during synthesis. An array comprises a surface with a collection of features, wherein the features comprise molecules or polymers attached to the surface. In certain embodiments of the invention, carrying out conditioning steps during array synthesis can yield arrays with improved signal. In other embodiments, linkers are synthesized on the array surface prior to synthesis of functional molecules, wherein increasing linker length can correspond to an improvement in the signal generated by the array.

Provided are methods for performing patterned chemistry and arrays prepared thereby.

Methods for performing patterned chemistry

Provided herein are methods and devices for performing in situ patterned chemistry for synthesizing and preparing peptide arrays. The invention provides a reproducible and scalable platform that can be potentially used to monitor the health of a plurality of individuals.

In situ chemical patterning

Non-covalent patterned chemical features and use thereof in MALDI-based quality control

Methods for synthetic antibodies, methods for making synthetic antibodies, methods for identifying ligands, and related methods and reagents.

Growing community of inventors

Tempe, AZ, United States of America

Neal W T Woodbury