W. R. Grace Co.-conn.

Infigen, Inc.

Neal L First

James M Robl

Michelle M Sims

Randall S Prather

Vincent F Simmon

Frank Barnes

Willard Eyestone

The use of totipotent embryonic stem cells to provide substantially identical cells for embryo cloning techniques is described. The method includes the culture of loose suspensions of inner cell mass cells of bovine animals to retrieve large populations of stem cells. The invention also describes the use of stem cells in various genetic manipulation techniques.

Culture of totipotent embryonic inner cells mass cells and production of

The disclosure herein describes a bovine embryo culture medium which includes epithelial cells from the oviduct of a donor animal. Additionally, a process for maturing immature bovine oocytes in an in vitro bovine culture medium containing epithelial cells from a donor oviduct is described. As an alternative to co-culture of the embryos with the epithelial cells, it is described how a culture medium can be conditioned for the culture of bovine embryos by the culture of epithelial cells therein which are later removed.

In vitro culture of bovine embryos

A method of multiplying bovine embryos by transfer of nuclei is described. The method includes enucleating a recipient oocyte, transferring the donor embryo nucleus to the enucleated recipient oocyte, and fusing the donor nucleus to the recipient oocyte by dielectrophoresis. The method is intended to allow the generation of multiplied genetically identical cattle.

Multiplying bovine embryos

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Madison, WI, United States of America

Neal L First