Riken Corporation

Natsuko Matsuda

Shigeyuki Yokoyama

Takanori Kigawa

Akiko Tanaka

Mikako Shirouzu

Namtip Chumpolkulwong

Chie Takemoto

It is provided a simple and efficient process for producing a large amount of protein in a cell-free protein synthesis system, through a continuous synthesis reaction over a long time using a linear DNA as a template. The continuous synthesis reaction is carried out for at least one hour with a cell-free protein synthesis system comprising an extract ofcells cultured at any temperature between 18&#xb0; C. and 36&#xb0; C., and a linear template DNA encoding the protein. The continuous protein synthesis system is preferably a dialysis system or a continuous flow system. The cell-free protein synthesis system by dialysis system comprises an internal dialysate comprising the extract and the linear template DNA, an external dialysate comprising a substrate solution for protein synthesis, and a dialysis membrane through which the substrate can be transferred.

Cell-free protein synthesis method with the use of linear template DNA and cell extract therefor

It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The above-described cells are preferably bacterial cells, in particular,cells. In the suppressed growth conditions as described above, the culture temperature is preferably from 20 to 32&#xb0; C., more preferably 26&#xb0; C. or higher and lower than 30&#xb0; C.

Process for producing extract for cell-free protein synthesis and cell extract produced thereby

Growing community of inventors

Yokohama, Japan

Natsuko Matsuda