Shimadzu Corporation

Natsuki Iwata

Minori Nakashima

Tomoko Yamaguchi

Azusa Uchida

A method for analyzing amino acids by liquid chromatography, in which a sample containing a plurality of kinds of amino acids is derivatized with a derivatization reagent, and the obtained derivatized sample is circulated on a column together with a mobile phase. Two or more kinds of derivatized samples are prepared using two or more kinds of derivatization reagents. Different analytical conditions in which mixing ratio of the plurality of the mobile phases is changed with a passage of time are set for each kind of the derivatization reagent. A solvent mixing ratio in the mobile phase being the mixed solvent system is set for the each kind of the derivatization reagent. The two or more kinds of derivatized samples are analyzed by automatically switching between the different analytical conditions and the solvent mixing ratio to separate and quantify derivatized L-amino acids and derivatized D-amino acids.

Amino acid analysis method

A method for analyzing coffee components includes, in this order, preparation step of preparing a sample solution containing coffee components extracted from coffee beans; dilution step of diluting the sample solution; and detection step of detecting the coffee components by performing liquid chromatography on the diluted sample solution. The detection step includes passing the sample solution through a column packed with a packing material having pentafluorophenylpropyl group, and then detecting trigonelline and pyrocatechol in the passed sample solution by a photodiode array ultraviolet-visible absorbance detector.

Method for analyzing coffee component

A sample is dissolved in a reagentcontaining an organic solvent in a conversion vessel. A gas is supplied from a first gas supply part into the conversion vesselvia a reagent introduction tube, and thus the interior of the conversion vesselis pressurized. A gas is supplied from a second gas supply part into the reagentin the conversion vesselvia a reagent discharge tube, and thus gas bubblesare formed in the reagent

Device for pretreatment of sample and analyzer equipped therewith, and method for pretreatment of sample

An analysis method for mycotoxins including a separation step, a detection step, and an identification step. In the separation step, each component contained in a liquid sample is separated in a column In the detection step, components separated in the separation step are detected by a PDA and a fluorescence detector. In the identification step, total aflatoxin is identified based on a detection signal from the fluorescence detector, and deoxynivalenol is identified based on a detection signal from the PDA.

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Kyoto, Japan

Natsuki Iwata