University of California

Nathaniel J Kingsbury

Karen A McDonald

Bryce William Falk

Abhaya M Dandekar

Benjamin E Lindenmuth

Minsook Hwang

Sang-Kyu Jung

The present disclosure relates to methods of increasing the extraction of an apoplast-targeted recombinant protein in a plant tissue and to methods of increasing the purity of an apoplast-targeted recombinant protein recovered from a plant tissue. The methods involve contacting plant tissue with a rinse fluid on a plurality of occasions to release recombinant protein from the apoplast into the rinse fluid to create an apoplast wash fluid. The resulting content of the recombinant protein in the apoplast wash fluid is higher than the content of the recombinant protein in the apoplast wash fluid from comparable plant tissue contacted with rinse fluid on a single occasion.

Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves

Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing anwith a Ti plasmid encoding a heterologous protein, infecting plant cells with the, allowing expression of the heterologous protein, and recovering the heterologous protein from the plant cells. In one embodiment, the protein produced is an endoglucanase.

Plant-based production of heterologous proteins

Growing community of inventors

Davis, CA, United States of America

Nathaniel J Kingsbury