Ventana Medical Systems, Inc.

Nathan Walter Polaske

Brian Daniel Kelly

Julia Ashworth-Sharpe

Christopher Bieniarz

Mark Lefever

Adrian E Murillo

Yuri Belosludtsev

Traci D DeGeer

Junshan Hao

Wendy J French

Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes 'click chemistry' to covalently bind reporter molecules to tissue.

Application of click chemistry for signal amplification in IHC and ISH assays

Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.

Colors for chromogenic IHC and ISH staining with multi-dye quinone methide and tyramide conjugates

Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Quinone methide analog signal amplification

Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.

Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens

Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens

Growing community of inventors

Oracle, AZ, United States of America

Nathan Walter Polaske