Affymetrix, Inc.

Covaris, Inc.

Delphax Systems

Electronics Corporation of America

Nabsys, 2.0 LLC

Genetic Microsystems, Inc.

Electronic Corporation of America

Nathan K Weiner

Eric E McKenzie

Mark R Jones

Christopher Miles

Patrick Odoy

Albert Bukys

David A Stura

Herman Deweerd

David Bradbury

Thomas P Regan

James T Overbeck

Roger DiPaolo

Joel M Katz

Tim J Woolaver

Peter D Honkanen

Eric Schultz

Timothy J Woolaver

Ksenia Oleink-Ovod

Akim Lennhoff

David D Ketchum

Jean I Montagu

Shantanu V Kaushikkar

Ksenia Oleinik-Ovod

Oleinik-Ovod Ksenia

Erik Schultz

Valentin Dimitrov

Gregory C Loney

John C Stephens

Peter J Weyman

Eric Earl Mckenzie

Patrick J Odoy

The invention relates to devices and methods for hybridization assisted sequencing of biomolecule analytes in nanopores, nano-channels and micro-channels. An alternating current signal may be employed to achieve a significantly improved signal-to-noise ratio, thereby enhancing system performance.

Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation

An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.

System and method for scanning of probe arrays

A system and method for controlling operation of a transducer circuit, e.g., including an acoustic transducer arranged to cause mixing, cavitation or other movement in a liquid contained in a vessel located remote from the transducer. A load current of a transformer used to generate a drive signal for the transducer may be controlled to vary sinusoidally, to vary between negative and positive values and such that the load current flows in a closed loop when a zero voltage bias is applied across the primary transformer winding, to have a reduced or eliminated 3harmonic mode and/or to vary between three discrete modes including a forward current mode, a zero voltage loop current mode, and a freewheel current mode. An inverter circuit may be used to control the load current, e.g., so that the load current flows in a closed loop during portions of the control cycle.

System and method for controlling an acoustic transducer

An embodiment of a method for adjusting system gain of a biological probe array scanner for a plurality of fluorophore species is described that comprises setting an excitation beam comprising an excitation wavelength at a first power level that elicits an optimal signal to noise ratio response from a first fluorophore species; scanning a biological probe array with the excitation beam; setting the excitation beam comprising the excitation wavelength at a second power level different than the first power level that elicits the optimal signal to noise ratio response from a second fluorophore species; and scanning the biological probe array with the excitation beam.

System, method, and product for multiple wavelength detection using single source excitation

System, method, and product for scanning of biological materials

Systems and methods are described for processing an emission signal, such as a fluorescent signal, to compensate for noise in an excitation beam, such as a laser beam. As one example, a scanning system is described that includes an excitation signal generator that provides an excitation signal having one or more representative excitation values representative of an excitation beam; an excitation reference provider that provides at least one excitation reference value; a normalization factor generator that compares the excitation reference value to at least one representative excitation value, thereby generating a normalization factor; and a comparison processor that adjusts at least one emission value corresponding to the at least one representative excitation value based, at least in part, on the normalization factor.

System, method, and product for dynamic noise reduction in scanning of biological material

A method for scanning a probe array is described. The method comprises tuning an excitation light to a plurality of wavelengths each within an excitation range of a fluorescent label associated with one or more target molecules; and directing the tuned excitation light of each wavelength at the probe array.

System, method, and product for providing a wavelength tunable excitation beam

Systems, methods, and computer program products are described for adjusting the gain of a scanner. The scanner includes one or more excitation sources, an emission detector having a first gain, and a variable gain element having a second gain. One described method includes receiving a user-selected gain value, adjusting the first gain based on a first portion of the user-selected gain value, and adjusting the second gain based on a second portion of the user-selected gain value. Another described method includes selecting an auto-gain value, adjusting the first gain based on a first portion of the auto-gain value, adjusting the second gain based on a second portion of the auto-gain value, causing the scanner to collect sample pixel intensity values, determining a comparison measure based on comparing the sample pixel intensity values to desired pixel intensity values, and adjusting the auto-gain value based on the comparison measure.

System, method, and computer software product for gain adjustment in biological microarray scanner

System, method, and product for dynamic noise reduction in scanning of biological materials

An apparatus is described that includes an emission signal detector and an emission signal filter. An excitation beam scans an array of biological materials, and the emission signal detector detects an emission signal indicative of an emission beam responsive to the excitation beam. The emission signal filter is a linear-phase filter that provides a filtered emission signal having substantially symmetrical rise and fall edges. A linear-phase excitation signal filter may also be provided that provides a filtered excitation signal having substantially symmetrical rise and fall edges. The emission signal filter and the excitation signal filter may be matched. In some applications, they may be high-order Bessel filters. Linear-phase filtering enables sampling of emission signals to be accomplished consistently irrespective of the scanning direction, and thus is particularly advantageous in bi-directional scanning applications.

System, method, and product for symmetrical filtering in scanning of biological materials

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Stoughton, MA, United States of America

Nathan K Weiner