University of California

Sandia Corporation

U.S. Department of Energy

Nathan J Hillson

Anup Kumar Singh

Gary L Andersen

Aarthi Chandrasekaran

Rajiv Bharadwaj

Lucille Shapiro

Ping Hu

Chieh Chang

The present invention provides a method of a method of designing an implementation of a DNA assembly. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding flanking homology sequences to each of the DNA oligos. In an exemplary embodiment, the method includes (1) receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments, (2) designing DNA oligonucleotides (oligos) for each of the DNA sequence fragments, and (3) creating a plan for adding optimized overhang sequences to each of the DNA oligos.

Scar-less multi-part DNA assembly design automation

This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.

Microfluidic platform for synthetic biology applications

Compositions and methods are provided for detection of certain heavy metals using bacterial whole cell biosensors.

Heavy metal biosensor

Growing community of inventors

San Francisco, CA, United States of America

Nathan J Hillson