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The University of Tennessee Research Foundation

Tranxenogen, Inc.

Nathan Howard Sloane

Alexander Baguisi

Paul DiTullio

Kathleen M Hehir

The invention provides compositions and methods for inhibiting angiogenesis associated with tumors and reducing tumor growth.

Anticancer peptide compositions

The invention provides a 16 amino acid peptide representing the partial N-terminal amino acid sequence of the Antineoplastic Protein (ANUP). The 16 amino acid peptide provided is a highly active pharmacologically antitumor agent.

Sixteen amino acid of the antineoplastic protein (ANUP) as a pharmacologically active anti-tumor agent

Electrophoretically homogeneous human Antineoplastic Urinary Protein (ANUP) contains a blocked N-terminal amino acid that has been identified as pyroglutamic acid. Removal of the pyroglutamy residue by the use of pyroglutamyl aminopeptidose results in the formation of the deblocked protein which is also an antineoplastic molecule. The amino acid sequence of the deblocked ANUP 16 KD monomer showed the following sequence: Cycle No.

Parital primary amino acid sequence of the antineoplastic protein

The passage of human urine through a bed of adsorbent material results in the adsorption from the urine of antineoplastic substances as determined by in vitro tissue culture techniques utilizing human tumor cells. The adsorbent yields two antineoplastic fractions upon sequential elution. Elution of the low-molecular weight antineoplastic fraction is accomplished by elution with cold aqueous acetone at slightly alkaline pH. Thereafter, elution of the high molecular weight antineoplastic fraction is accomplished by elution with a cold aqueous acetone-glycerol mixture at slightly alkaline pH. The antineoplastic activities of these fractions are determined by tissue culture techniques employing a variety of human neoplastic cells. Furthermore, the high molecular weight fraction, the antineoplastic urinary protein, also inhibits the progression and causes regression of certain human tumor cells implanted in the nude mouse.

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Germantown, TN, United States of America

Nathan Howard Sloane