Illumina Cambridge Limited

Illumina Corporation

Natalie Morrell

Andrew Slatter

Grace DeSantis

Samantha Snow

Jian-Sen Li

Stephen M Gross

Kevin Shen

Niall Anthony Gormley

Rosamond Jackson

Avgousta Ioannou

Kevin L Gunderson

Molly Min He

Frank J Steemers

Amirali Kia

Igor Yu Goryshin

Jason Richard Betley

Rigo Pantoja

Dmitry K Pokholok

Fan Zhang

Wouter Meuleman

Gareth Jenkins

Vicki Thomson

Jacqueline Weir

Steven J Norberg

Igor Goryshin

The present disclosure relates to methods, compositions, and kits for treating target nucleic acids, including methods and compositions for fragmenting and tagging nucleic acid (e.g., DNA) using transposome complexes bound to a solid support.

Tagmentation using immobilized transposomes with linkers

Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

Contiguity preserving transposition

In an example method, a series of time-based clustering images is generated for a plurality of library fragments from a genome sample. Each time-based clustering image in the series is sequentially generated. To generate each time-based clustering image in the series: i) a respective sample is introduced to a flow cell, the respective sample including contiguity preserved library fragments of the plurality of library fragments, wherein the contiguity preserved library fragments are attached to a solid support or are attached to each other; ii) the contiguity preserved library fragments are released from the solid support or from each other; iii) the contiguity preserved library fragments are amplified to generate a plurality of respective template strands; iv) the respective template strands are stained; and v) the respective template strands are imaged.

Time-based cluster imaging of amplified contiguity-preserved library fragments of genomic DNA

Presented herein are methods and compositions surface-based tagmentation. In particular embodiments, methods of preparing an immobilized library of fragmented and tagged DNA molecules on a solid surface are presented. In particular embodiments, the solid surface comprises immobilized transposomes in a dried format, suitable for reconstitution upon contact with liquid, such as a liquid sample.

Surface-based tagmentation

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Saffron Walden, United Kingdom

Natalie Morrell