E.i. Dupont De Nemours and Company

Pioneer Hi-Bred International, Inc.

Natalie M Keiper-Hrynko

David L Hallahan

This invention pertains to nucleic acid fragments encoding plant cis-prenyltransferases. More specifically, this invention pertains to a cis-prenyltransferase homolog from latex of the rubber-producing plant species-(russian dandelion) and a cis-prenyltransferase homolog from the rubber-producing plant species(sunflower).

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The instant invention relates to bioproduction of isopentenyl diphosphate within the latex of the rubber tree,. Isopentenyl diphosphate (IPP) synthesis from acetate is mediated by a set of enzymes, acetyl-coA acetyltransferase, HMG-coA synthase, HMG-coA reductase, mevalonate kinase, phosphomevalonate kinase and mevalonate diphosphate decarboxylase, encoded by six genes isolated as expressed sequence tag (EST) cDNAs fromlatex. In addition, a variant sequence of one of these enzymes, acetyl-coA acetyltransferase, has been found.

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Bear, DE, United States of America

Natalie M Keiper-Hrynko