International Business Machines Corporation

The University of Melbourne

The Florey Institute of Neuroscience and Mental Health

Natalie J Gunn

John Michael Wagner

Priscilla Racquel Rogers

Stefan Harrer

Jose Miguel Lobez Comeras

Matthew Downton

Ross Bathgate

Daniel Scott

Stan Skafidas

Ali Afzali-Ardakani

Julia S Baldauf

Christine Schieber

Sung Cheol Kim

Sridhar Kannam

Matthew Downtown

Jose M Lobez Comeras

A process for monitoring binding events, a coating compound, and a device for monitoring binding events are disclosed. The process includes providing two reservoirs that contain an electrically conductive fluid, wherein the reservoirs are separated by a membrane having a nanopore. The process also includes selecting a target compound, selecting a ligand based on the selection of the target compound, and preparing a coating that includes the coating compound, wherein the coating compound includes the ligand and a moiety that immobilizes the ligand. Additionally, the process includes applying the coating to the inner surface of the nanopore, adding the target compound to a conductive liquid in the first chamber, establishing a voltage gradient across the membrane, and electrically monitoring translocation of the target molecule. The device includes the reservoirs and the membrane having the coated nanopore.

Nanopore coating for sensing chemical bond formation

A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.

Use of disulfide bonds to form a reversible and reusable coating for nanofluidic devices

Translocation events are sensed using composite nanopore assemblies including nanopores formed in graphene sheets. Single molecule detection and characterization and multi-molecule characterization and identification are provided using such assemblies. Multiple electrodes associated with nanofluidic sensors facilitate detection of ionic current through a nanopore as well as tunneling currents. Current signals of individual molecules are estimated from the combination of an ionic current signal through the nanopore and tunneling current signals obtained at specific locations within the nanopore.

Detection of translocation events using graphene-based nanopore assemblies

A mechanism is provided for utilizing a nanodevice to distinguish molecules with different structure. The molecules translocate through or across a nanochannel filled with a electrolyte solution. An electrical signal through the nanochannel is measured for every translocation event. Inner surfaces of the nanochannel include a functional layer, which is a coating to functionalize the nanochannel, in which the functional layer is configured to interact with predetermined ones of the molecules during translocation events. It is determined that a combination of at least two different molecules is formed based on predetermined ones of the molecules interacting with the functional layer to change the electrical signal and/or change a translocation time for the translocation event.

Methods for determining binding capability of target ligands with G protein-coupled receptors using translocation through nanochannels

Modifying single proteins (GPCR), ligands, and nanopore surfaces to create binding-induced molecular changes of protein-ligand complexes detected in nanochannel translocation

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Eltham, Australia

Natalie J Gunn