Third Wave Technologies, Inc.

Natalie E Lyamicheva

Hatim T Allawi

Victor I Lyamichev

Bruce P Neri

Michael W Kaiser

James J Schaefer

Jeff G Hall

Peggy S Eis

Lin Ji

Zbigniev Skrzypczynski

Sarah Olson

Andrew A Lukowiak

Marilyn C Olson-Munoz

LuAnne Chehak

Hon Ip

Michelle Curtis

Tsetska Y Takova

Christian Tor Bartholomay

WuPo Ma

Kevin L Vedvik

Lisa C Thompson

Brad T Argue

Robert W Kwiatkowski, Jr

Wu-Po Ma

Robert W Kwiatkowski

Michelle L Curtis

Hon S Ip

Sarah M Olson

Jr Robert W Kwiatkowski

Bruce N Neri

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

RNA detection assays

The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.

Enzymes for the detection of specific nucleic acid sequences

Enzymes for the detection of nucleic acid sequences

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Madison, WI, United States of America

Natalie E Lyamicheva