Berkeley Lights, Inc.

Bruker Cellular Analysis, Inc.

Bruker Cellular Analysic, Inc.

Natalie C Marks

J Tanner Nevill

Mark P White

Daniele Malleo

Andrew W McFarland

Aathavan Karunakaran

Randall D Lowe, Jr

Kevin T Chapman

Jason M McEwen

Xiao Guan

Keith J Breinlinger

Alexander J Mastroianni

Gang F Wang

Xiaohua Wang

Kristin G Beaumont

Peter J Beemiller

Keith Joseph Breinlinger

Guido K Stadler

Shao Ning Pei

Yelena Bronevetsky

Hansohl E Kim

Thomas M Vetterli

Vincent Haw Tien Pai

Kevin D Loutherback

Annamaria Mocciaro

Jason M Mcewen

Amanda L Goodsell

Duane Smith

Jr Randall D Lowe

Andrew W Mcfarland

Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Methods for assaying biological cells in a microfluidic device

Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Microfluidic cell culture

Growing community of inventors

Albany, CA, United States of America

Natalie C Marks