Abbott Laboratories Corporation

Abbott Molecular Inc.

Natalie A Solomon

Stanley R Bouma

Julian D Gordon

John J Carrino

Edward N Granados

Matthew T Manlove

Peter Zaun

Ingrid E Semple-Facey

Alan H Davis

Gregor W Leckie

Eric B Shain

Larry G Birkenmeyer

Gerard J Gundling

Jon D Kratochvil

Lisa A Roberts-Rapp

Ronald L Marshall

John J Kotlarik

Ronald A Coules

Bruce J Dille

Wai-Bing X Mak

Thomas G Laffler

Hsiang-Yun Y Hu

Laurie A Rinehardt

Magdalena Szostak

Robert Kowal

The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating low molecular weight circulating nucleic acids from bodily fluids (e.g., plasma).

Systems and methods for isolating nucleic acids

Polynucleotides are provided which are useful as amplification primers and hybridization probes for detecting BS106 target sequence in a test sample. The primers and probes can be employed in amplification based methods for detecting the presence of BS106 sequences in a test sample. Additionally, the primers and probes can be used to perform homogeneous, real time reverse-transcriptase polymerase chain reaction to detect BS106 target sequence in a test sample.

Nucleic acid primers and probes for detecting breast cells

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction/detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.

Apparatus and method for transfer of a fluid sample

Provided herein are methods for detecting multiple target nucleic acid sequences in a test sample. Also provided is a hybridization platform useful for detecting multiple target sequences in a test sample. The hybridization platform comprises a solid support material having a defined pattern of capture probes immobilized thereon.

Method and reagent for detecting multiple nucleic acid sequences in a

The present invention provides a method of detecting the amount of a target sequence which may be present in a test sample. The method uses an aggregate primer series, which comprises at least two primer sets, in an amplification reaction to detect the relative concentration of a target sequence which may be present in a test sample. The primer sets have different sensitivities and hybridize with sub-target sequences which are different regions of the target sequence. The method generally comprises cycling a test sample suspected of containing a target sequence, an aggregate primer series, and means necessary for performing an amplification reaction; and detecting any amplified sub-target sequences. Based on a qualitative detection of the amplified sub-target sequences generated by individual primer sets, the relative quantity of the target sequence can be determined.

Wide dynamic range nucleic acid detection using an aggregate primer

The present invention is directed to oligonucleotides useful in detection, e.g., by ligase chain reaction (LCR) of target DNA from Mycobacterium tuberculosis. The present invention is also directed to methods of detecting target DNAs from Mycobacterium tuberculosis.

Materials and methods for the detection of mycobacterium tuberculosis

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection chamber to form a sealed reaction/detection unit that is virtually irreversibly closed. One or more heating elements of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Kits include the reaction/detection units and reagents for amplification.

Device and method for amplifying and detecting target nucleic acids

Materials and methods for the detection of Mycobacterium tuberculosis

An improved, 'gap filling' embodiment of the Ligase Chain Reaction (LCR) is described. Gap filling LCR is LCR wherein at least one of the probes is recessed so that a gap is formed between the adjacent probes when they are hybridized to target. The gap is filled using polymerase and deoxyribonucleotide triphosphates before ligation of the probes together. There are single and double gap versions, depending on whether one or two probes are recessed and require filling before ligation. The improvement resides in selecting and using target sequences such that only a single type, or two types, of deoxyribonucleotide triphosphate(s) are required to fill double gaps each being 1-10 bases in length, preferably 1-3 bases. Probes having specific sequences are claimed for a number of pathogens.

Amplification of target nucleic acids using gap filling ligase chain

Growing community of inventors

Buffalo Grove, IL, United States of America

Natalie A Solomon