Other

Biotecnol S.a.

Natalia Battchikova

Timo Korpela

Anton Zavialov

Vladimir Zav'yalov

Olga Shulga

Maria Kouznetsova

Konstantin Skryabin

Vitaly Dzhavakhia

Tatiana Voinova

Mikhail Pridanniko

Daria Shumilina

Sheila Macintyre-Ayane

Lada Petrovskaya

Alexei Filippov

Ksenia Kromina

Galina Petrovna Korobko

The invention relates to isolated DNA sequences encoding MF3 polypeptides that induce multiple resistance in plants toward a variety of viral and microbial infections and against pests and a method of obtaining transgenic plants expressing MF3 polypeptides. The invention also relates to a method of isolating and purifying an MF3 polypeptide from bacterial cells expressing the polypeptide and its use as a plant protectant with or without carrier agent.

Isolated DNA sequences and polypeptides inducing multiple resistance of plants to phytopathogens and pests

The present invention provides bacterial strains for secretion of soluble biologically active recombinant heterologous proteins into periplasm or on a surface/into a cultivation medium of bacteria. The invention exploits the secretion system of Gram-negative bacteria including periplasmic chaperones and usher/secretin proteins. For accomplishing the aim, the bacterial strains simultaneously express the fusion protein (signal peptide)-(mature hecterologous protein)-(subunit of a bacterial surface structure, Caf1), periplasmic chaperone specific for the subunit, and outer membrane usher/secretin protein specific for the subunit. Secretion of fusion proteins: (signal peptide of Caf1)-(mature human IL-1&#x3b2;)-(mature Caf1), (signal peptide of Caf1)-(mature human GM-CSF)-(mature Caf1), and (signal peptide of Caf1)-(mature human IL-1ra)-(mature Caf1) that were expressed insimultaneously with the periplasmic chaperone Caf1M and the usher/secretin protein Caf1A are examples of the use of the invention.

Microbial protein expression system

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Turku, Finland

Natalia Battchikova