The Texas A&m University System

The Uab Research Foundation

Inhibitex, Inc.

Narayana Sthanam

Joseph M Patti

Magnus A Hook

Jeff T Hutchins

Yi Xu

Andrea Hall

Maria G Bowden

Jouko V Sillanpaa

Karthe Ponnuraj

Karen L House-Pompeo

Magnus H&ouml;&ouml;k

Jindrich Symersky

A bioinformatic method for identifying and isolating proteins and peptides with MSCRAMM&#xae;-like characteristics from Gram positive bacteria, such asandbacteria, and proteins and peptides obtained thereby are provided which can be utilized in methods to prevent and treat infections caused by Gram-positive bacteria. The method involves identifying from sequence information those proteins with a putative C-terminal LPXTG (SEQ ID NO:1) cell wall sorting signal and other structural similarities to MSCRAMM&#xae; proteins having the LPXTG-anchored cell wall proteins. The MSCRAMM&#xae; proteins and immunogenic regions therein that are identified and isolated using the present invention may be useful in the diagnosis, treatment or prevention of Gram positive bacterial infections.

Bioinformatic method for identifying surface-anchored proteins from gram-positive bacteria and proteins obtained thereby

A bioinformatic method is provided for identifying and isolating proteins with MSCRAMM&#xae;&#x2014;like characteristics from Gram positive bacteria, such asandbacteria, which can then be utilized in methods to prevent and treat infections caused by Gram-positive bacteria. The method involves identifying from sequence information those proteins with a putative C-terminal LPXTG (SEQ ID NO:1) cell wall sorting signal and other structural similarities to MSCRAMM&#xae; proteins having the LPXTG-anchored cell wall proteins. The MSCRAMM&#xae; proteins and immunogenic regions therein that are identified and isolated using the present invention may be used to generate antibodies useful in the diagnosis, treatment or prevention of Gram positive bacterial infections.

Disclosed are the cna gene and cna-derived nucleic acid segments from,, and DNA segments encoding cna from related bacteria. Also disclosed are Col binding protein (CBP) compositions and methods of use. The CBP protein and antigenic epitopes derived therefrom are contemplated for use in the treatment of pathological infections, and in particular, for use in the prevention of bacterial adhesion to Col. DNA segments encoding these proteins and anti-(Col binding protein) antibodies will also be of use in various screening, diagnostic and therapeutic applications including active and passive immunization and methods for the prevention of bacterial colonization in an animal such as a human. These DNA segments and the peptides derived therefrom are contemplated for use in the preparation of vaccines and, also, for use as carrier proteins in vaccine formulations, and in the formulation of compositions for use in the prevention of,infection.

Collagen binding protein compositions and methods of use

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Birmingham, AL, United States of America

Narayana Sthanam