Wako Pure Chemical Industries, Inc.

Fujifilm Wako Pure Chemical Chemical Corporation

Naoyuki Yamamoto

Satoshi Hasaba

Tatsuo Kurosawa

Toshinari Ohashi

Matsuhiro Date

Yukinobu Hayashida

Isao Koyama

Yo Yagura

Mutsuhiro Date

Makoto Amano

Tsutomu Masuda

Takeshi Kitagawa

Yoshikazu Fujita

Tomokazu Itai

Shinya Kaji

Takashi Takagaki

Kiyoko Mori

The invention relates to a stabilizer for L-012 or a salt thereof, a stabilization method for L-012 or a salt thereof, coexisting with the stabilizer and L-012 or a salt thereof, and the like. The stabilizer is represented by Formula [1]: wherein p pieces of M1 each independently represent a hydrogen atom or an alkali metal atom, q pieces of R1 each independently represent a hydroxy group or a sulfonic acid group, m represents 0 or 1, p represents an integer of 1 to 3, q represents an integer of 0 to 4, Y represents a nitrogen atom or a CH group (a methine group), and Z represents an aryl group having a specific structure or a pyrazolyl group having a specific structure.

Stabilizer and stabilization method

The purpose of the present invention is to provide an agglutination enhancer which shows superior agglutination enhancing effect to those of conventional immunoagglutination enhancers, and the present invention relates to an agglutination enhancer for an immunoagglutination measurement method which comprises a polymer having a monomer unit shown by the following general formula [1]: (Wherein Rrepresents a hydrogen atom or a methyl group; Rand Rindependently represent a methyl group or an ethyl group, respectively; X represents &#x2014;NH&#x2014; or an oxygen atom; n represents an integer of 1 to 6; and m represents an integer of 1 to 3), and an immunoagglutination measurement method in which, in the coexistence of the above-described agglutination enhancer for immunoagglutination measurement method, an antibody against analyte or an antigen for the analyte is brought into contact with the analyte to cause an antigen-antibody reaction.

Agglutination enhancer

The invention provides a method for converting non-methylated cytosine in a single-stranded DNA into uracil by a bisulfite reaction with a high conversion efficiency from non-methylated cytosine into uracil. The invention also provides a method for amplifying the single-stranded DNA in which non-methylated cytosine has been converted into uracil, as well as a method for detecting methylated cytosine in the single-stranded DNA.

Method for detecting methylated cytosine by using bisulfite reaction

The present invention addresses the problem of providing a chelate color former which can measure the concentration of iron with high sensitivity and with low blank values when measuring the concentration of iron, and an iron concentration measurement method and kit that use this chelate color former. The present invention relates to: a compound represented by following formula [1] or a salt thereof wherein, Rand Reach independently represent &#x2014;SOH or &#x2014;COH; an iron concentration measurement method in which the compound is used as a chelate color former, the compound is brought into contact with iron in a sample, and the concentration of iron in the sample is measured on the basis of the degree of resulting color development; and a kit to be used therein.

Compound, and method for measuring iron concentration by using novel compound as chelate color former

PROBLEM Provided is a novel cyanine dye derivative with a pyrazole skeleton and an indole skeleton, having high sensitivity performance in a shorter wavelength region as compared with a conventional optical system, and showing high water solubility. SOLUTION The invention provides (1) a compound represented by the following general formula [50] and a salt thereof: [wherein Rto Reach independently represent a substituted or unsubstituted alkyl group which may have an amide bond; Rto Reach independently represent alkyl group, alkenyl group, alkynyl group, aryl group, alkoxy group, aryloxy group, alkylthio group, arylthio group, alkylsulfonyl group, arylsulfonyl group, carbamoyl group, sulfamoyl group, ureido group or amino group, those groups being able to have substituents; a group represented by the general formula [2]:&#x2014;COOR&#x2003;&#x2003;[2](wherein Rrepresents hydrogen atom, Cto Calkyl group, alkali metal atom, organic ammonium ion, ammonium ion or anion); a group represented by the general formula [3]:&#x2014;SOR&#x2003;&#x2003;[3](wherein Rrepresents hydrogen atom, alkali metal atom, organic ammonium ion, ammonium ion or anion), halogen atom, aromatic heterocyclic thio group, hydrogen atom, hydroxyl group, cyano group, formyl group, thiol group or nitro group; Rrepresents hydrogen atom, or alkyl group, alkenyl group, alkynyl group or aryl group, those groups being able to have substituents; and n represents an integer of from 0 to 3, provided that any of Rand R, Rand R, Rand R, and Rand Rmay form a bivalent group with a group selected from &#x2014;O&#x2014; group, &#x2014;S&#x2014; group, &#x2014;COO&#x2014; group and groups represented by the general formulae [52] to [54]: (wherein Rrepresents hydrogen atom, alkyl group, alkenyl group or aryl group, those groups being able to have substituents), and substituted or unsubstituted alkylene group; and in the case where said bivalent group is formed, at least one of Rto R, along with the bivalent group formed by any of Rand R, Rand R, Rand R, and Rand R, has the group represented by the general formula [2], the group represented by the general formula [3], amino group, hydroxyl group, thiol group or formyl group; and in the case where said bivalent group is not formed, at least one of Rto Rhas the group represented by the general formula [2], the group represented by the general formula [3], amino group, hydroxyl group, thiol group or formyl group]; (2) a labeled compound obtained by subjecting the above compound to direct or indirect binding to a substance to be labeled, and (3) a method for labeling a substance to be labeled, comprising subjecting the above compound to direct or indirect binding to the substance to be labeled.

Pyrazole-based cyanine dye

A subject of the present invention is to provide a measurement method using an internal standard substance in an electrophoresis where an analyte is a protein or a compound. The present invention relates to a measurement method for an analyte by an electrophoresis, characterized in that a peak of the analyte is identified by using as an internal standard substance (1) a combination of a compound I having 3 or more anion groups in a molecule and a compound II where 1 to 3 groups of the anion groups of said compound I have been substituted by cation groups, or (2) a combination of a compound III having 3 or more cation groups in a molecule and a compound IV where 1 to 3 groups of the cation groups of said compound III have been substituted by cation groups.

Measurement method utilizing internal standard substance

A cyanine dye derivative having high fluorescence intensity in a short wavelength region, and also a controllable migration velocity by further introducing a quaternary ammonium cation. A compound having general formula [1] or a salt thereof: wherein Rto Reach independently represent an alkyl group having, as a substituent, a group represented by the general formula [101]: 

Pyrazole-based cyanine dye containing quaternary ammonium cation

An object of the present invention is to provide a peptide derivative for determining &#x3b2;-glucan or endotoxin which allows high sensitivity measurement, and a method for determining &#x3b2;-glucan and/or endotoxin using the same. The present invention relates to (1) a peptide derivative represented by the following general formula [1]:X-A-Gly-Arg-A-E-D&#x2003;&#x2003;[1],(2) a reagent for determining &#x3b2;-glucan and/or endotoxin comprising the above-described peptide derivative, (3) a method for determining &#x3b2;-glucan and/or endotoxin, characterized in that a sample containing &#x3b2;-glucan and/or endotoxin, an amebocyte lysate of a horseshoe crab and the above-described peptide derivative are reacted each other, then the resulting released compound represented by the following general formula [2]:H-A-E-D&#x2003;&#x2003;[2]is separated from unreacted substance and quantified, and the determination is made based on this value, and (4) a reagent kit for determining &#x3b2;-glucan and/or endotoxin, comprising an amebocyte lysate of a horseshoe crab and the above-described peptide derivative as constituents thereof.

Substrate for assaying &#x3b2;-glucan and/or endotoxin and assay method

It is an object of the present invention to provide the method for determining cholesterol in lipoprotein and reagent for determining cholesterol comprising the polymer, and the present invention relates to the reagent for determining cholesterol in lipoproteins such as high-density lipoprotein (HDL), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) and the determination method using the polymer comprising the following units as constituents: (iv) a polyethylene glycol segment represented by the general formula [1]:&#x2014;(CHCHO)&#x2003;&#x2003;[1](wherein, k represents an integer of 10 to 250);(v) a monomer unit represented by the general formula [2]: (wherein Rrepresents a hydrogen atom or C-Calkyl group); and(vi) a monomer unit represented by the general formula [3]: [wherein, Rrepresents a hydrogen atom or C-Calkyl group, and Rrepresents a group represented by the general formula [4]:&#x2014;COOR&#x2003;&#x2003;[4](wherein, Rrepresents an alkyl group, a haloalkyl group or a bornyl group), an alkyl group, an alkoxy group, an aralkyl group or an alkylcarbamoyl group; particularly, in the direct measurement of cholesterols such as HDL, LDL and VLDL contained in the sample, the reagent for determining cholesterol in the presence of said polymer, and the method for determining the concentration of cholesterol in a specific lipoprotein using said reagent for determining cholesterol, and a novel polymer.

Polymer and method of measuring cholesterol therewith

[PROBLEMS] To provide a CGH method, in particular a CGH microarray method, enabling detection at an elevated accuracy and elevated sensitivity. [MEANS FOR SOLVING PROBLEMS] A method which comprises: (a) labeling a test genomic DNA fragment that is a cell-origin genomic DNA fragment to be tested with either a label represented by the following general formula (1) or another label represented by the general formula (2), and labeling a control genomic fragment that serves as a standard for detecting a difference from the test genomic DNA fragment with the other label; (b) hybridizing the labeled test genomic DNA fragment and the labeled control genomic DNA fragment each with a specimen nucleic acid containing a nucleic acid sequence for detecting the difference between the test genomic fragment and the control genomic fragment; and (c) detecting an amplification or deletion in the test genomic DNA fragment by using the fluorescent intensities thus obtained as an indication; and a kit for the above-described method which contains a nucleotide residue labeled with the label represented by the following general formula (1) and a nucleotide residue labeled with the label represented by the following general formula (2).

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Amagasaki, Japan

Naoyuki Yamamoto