Takara Shuzo Co., Inc.

Osaka University

Toyo Boseki Kabushiki Kaisha

Suntory Limited

Ube Industries, Inc.

Suntory Holdings Limited

Wako Pure Chemical Industries, Inc.

Takara Bio Inc.

Kochi University

Naoyuki Taniguchi

Eiji Miyoshi

Atsushi Nishikawa

Yoshito Ihara

Tetsuo Shiba

Shusaku Yanagidani

Naofumi Uozumi

Nobuto Koyama

Nozomi Yamaguchi

Akihiro Kondo

Shunichiro Taniguchi

Takatoshi Nakagawa

Masafumi Yoshimura

Ikunoshin Kato

Takashi Saito

Taizo Uda

Tetsuo Kawaguchi

Yukikatsu Itoh

Wenzhe Li

Koichi Honke

Norihiro Kotani

Mutsuo Ishikawa

Shirou Noji

Keiichiro Suzuki

Emi Hifumi

Hideki Iijima

Shinichiro Shinzaki

Masahiko Tsujii

Norio Hayashi

Miyako Nakano

The present invention aims to provide a convenient and low-cost method for detection of a wide variety of compounds interacting with a target molecule located on a cell membrane, using a living cell without need of separating the cell membrane or the like from the cell. The present invention also aims to provide a kit for carrying out the method of the present invention. The method for detection of the compound interacting with the molecule located on the cell membrane in the present invention comprises steps of, allowing a compound having a moiety capable of binding selectively to the molecule located on the cell membrane and a radicalization-promoting moiety, to act on the cell; further allowing a compound having a group capable of being radicalized by the radicalization-promoting moiety and a labeling group, to act on the cell; and identifying the interacting compound bound by the compound radicalized by the radicalization-promoting moiety.

Method for detection of compound interacting with molecule located on cell membrane

The instant invention provides a method for the differential diagnosis of inflammatory bowel disease which comprises, determining the relative ratio of G0 oligosaccharide represented by formula (I): to G2 oligosaccharide represented by formula (II): wherein G represents galactose, M represents mannose, GN represents N-acetylglucosamine and F represents fucose in serum IgG oligosaccharide fraction obtained from a patient with inflammatory bowel disease or a patient with suspicion of inflammatory bowel disease, anddiscriminating inflammatory bowel disease based on the obtained ratio.

Method for determination of inflammatory bowel disease

Disclosed is a means for reducing the expression amount of a FUT8 gene, reducing the expression amount of a FUT8 protein, and/or reducing the expression amount of a product produced by the action of FUT8.

Composition for suppressing the expression of fucosyltransferase

The determination (test, diagnosis) of pancreatic cancer can be performed with high accuracy by detecting a fucosylated sugar chain (N-glycan) present in a specific site of the human haptoglobin and using an amount of the fucosylated sugar chain as a tumor marker for pancreatic cancer.

Tumor marker for pancreatic cancer and method of testing the same

The present invention provides a peptide or protein having a neovascularization action and containing a basic amino acid cluster region of &#x3b2;1,6-N-acetylglucosaminyltransferase, a neovascularization accelerator containing the above-mentioned peptide or protein, a method of screening an inhibition substance for the above-mentioned peptide or protein, and a neovascularization inhibitor containing this inhibition substance.

Glycosyltransferase GnT-V having neovascularization action

An isolated, porcine &#x3b1;1-6 fucosyltransferase having the following physico-chemical properties:

&#x3b1;1-6 fucosyltransferase

&agr;1-6 fucosyltransferase

Inhibitor for viral replication

.alpha.1-6 fucosyltransferase

A gene encoding human glycosyltransferase (human GnT-III) and recombinant DNA method for producing the enzyme are provided. A cancerous metastasis inhibitor comprising GnT-III, or a gene thereof, and a method of inhibiting cancerous metastasis in a mammal are also provided.

Human glycosyltransferase gene, compounds and method for inhibiting

A .beta.1,6-N-acetylglucosaminyl transferase having the following properties: (1) Action: it transfers N-acetylglucosamine from UDP-N-acetylglucosamine to .alpha.-6-D-mannoside; (2) Substrate specificity: it shows a reactivity of about 79% for GnGnF-bi-PA, about 125% for GnGnGn-tri-PA and about 66% for GnM-Pa, when taking a reactivity for GnGn-bi-PA as 100%; (3) Optimum pH: 6.2 to 6.3; (4) Inhibition, Activation and Stability: Mn.sup.2+ is not necessary for expression of activity, and the activity is not inhibited in the presence of 20 mM EDTA; (5) Molecular weight: about 73,000 as determined by SDS-PAGE in the absence of reducing agent; and about 73,000 and about 60,000 as determined in the presence of a reducing agent; (6) Km value: 133 .mu.M and 3.5 mM for acceptor GnGn-bi-PA and donor UDP-GlcNAc, respectively; and (7) It includes the following peptide fragments:

N-acetylglucosaminyl transferase gene coding therefor and process for

An isolated DNA having a promoter activity in an animal cell; a method for expressing a useful gene using the isolated DNA; and a method for producing a protein in an animal cell using the isolated DNA. The present invention provides a method for producing a desired gene product in a large quantity in an animal cell.

Promoter and method of gene expression using the same

A monoclonal antibody against human manganese-superoxide dismutase characterized in that it is produced by a cell line which has been obtained by immunization of a mouse with human Mn-SOD and then fusion of lymphocytes obtained from the mouse with mouse myeloma cells, and has high specificity against human Mn-SOD; a method for producing a monoclonal antibody against human Mn-SOD having high specificity against human Mn-SOD, which comprises culturing the cell line obtained by immunization of a mouse with human Mn-SOD and then cell fusion of lymphocytes obtained from the mouse with mouse myeloma cells; a kit for assaying human Mn-SOD, comprising:

Methods to screen for ovarian cancer and myocardial infarction

Growing community of inventors

Toyonaka, Japan

Naoyuki Taniguchi