The Green Cross Corporation

Naoto Fuluhata

Takao Ohmura

Akinori Sumi

Wataru Ohtani

Kazumasa Yokoyama

Kaoru Kobayashi

Munehiro Noda

Shinobu Kuwae

Kaeko Kamide

Tomoshi Ohya

Hiroshi Morise

Kazuya Takeshima

Kazuhiro Oohara

Syoichi Ishikawa

Masahide Kondo

Hirotoshi Fukutsuka

Method for highly purifying human serum albumin

Human serum albumin obtained by gene manipulation techniques can be purified by a combination of specified steps in which a culture supernatant obtained from a human serum albumin-producing host is subjected to ultrafiltration, heat treatment, acid treatment and another ultrafiltration, followed by subsequent treatments with a cation exchanger, a hydrophobic chromatography carrier and an anion exchanger, and by salting-out to thereby obtain a pure form of human serum albumin which contains substantially no proteinous and polysaccharide contaminants, which is formulated into a pharmaceutical preparation. This process makes it possible to effeciently purify recombinant human serum albumin and to provide substantially pure human serum albumin which does not contain producer host-related substances and other contaminants and is sufficiently free from coloration.

Recombinant human serum albumin, process for producing the same and

A method of inhibiting the coloration of human serum albumin expressed by using the gene manipulation technology which method comprises separating coloring contaminants from said human serum albumin before said coloring contaminants bind to the human serum albumin.

Inhibition of coloration of human serum albumin

Growing community of inventors

Osaka, Japan

Naoto Fuluhata