National Institute of Advanced Industrial Science and Technology

National Institute of Technology and Evaluation

National Research Institute of Brewing

Kao Corporation

Nara Institute of Science and Technology

Amano Enzyme Inc.

Naotake Ogasawara

Kiyoshi Asai

Masayuki Machida

Keietsu Abe

Katsutoshi Ara

Shotaro Yamaguchi

Katsuya Ozaki

Motoaki Sano

Katsuya Gomi

Hideki Nagasaki

Osamu Akita

Taishin Kin

Akira Hosoyama

Satoru Kuhara

Yasuji Koyama

Keiji Endo

Masatoshi Tohata

Kazuhisa Sawada

Katsuhiko Kitamoto

Kotaro Ito

Atsuki Toumoto

Kenichiro Matsushima

Kensuke Yuuki

Takuya Morimoto

Manabu Arioka

Ryosuke Kadoya

Satoru Hisahara

Misao Sunagawa

Tasuku Nakajima

Kazuo Kobayashi

Hideki c/o Aist Tokyo Waterfront Nagasaki

Shotaro c/o Gifu R & D Center Yamaguchi

Motoaki c/o Tsukuba Central 6 Sano

Masayuki c/o Tsukuba Central 6 Machida

Kiyoshi c/o Aist Tokyo Waterfront Asai

Taishin c/o Aist Tokyo Waterfront Kin

A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5'-side region outside of the target region in the host DNA, a 3&#x2032;-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3&#x2032;-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5&#x2032;-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3&#x2032;-side region derived from the host DNA and the 3&#x2032;-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter; and a selectable marker cassette for use in the method.

Method of modifying target region in host DNA and selectable marker cassette

Novelmutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived fromvia gene disruption. Themutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of thesemutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.

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It is intended to provide koji mold-origin phospholipase Aand a DNA encoding it. Namely, phospholipase Acomprising the following protein (a) or (b): (a) a protein having an amino acid sequence represented by SEQ ID NO: 1 or 2; and (b) a protein having an amino acid sequence derived from an amino acid sequence represented by SEQ ID NO: 1 or 2 by a partial modification and serving as phospholipase A.

Lysyl oxidase derived from filamentous fungi and a DNA encoding thereof are provided. Lysyl oxidase including a protein described in of the following (a) or (b): (a) a protein having an amino acid sequence set forth in SEQ ID NO: 2; or (b) a protein having an amino acid sequence obtained by modifying a part of the amino acid sequence set forth in SEQ ID NO: 2, and functioning as lysyl oxidase.

Fungus-origin lysyl oxidases

The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-&#x3b2;--glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade &#x3b2;--glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having &#x3b2;--glucanase activity (exo-, or endo-&#x3b2;--glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having &#x3b2;--glucanase activity, and a method for the production of said enzymes.

Genes of cell wall-degrading enzymes derived from aspergillus, and method for the production the enzymes

A completely novel glutaminase is provided: (a) a protein consisting of an amino acid sequence represented by SEQ ID NO: 2, or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one or more amino acids, and possessing glutaminase activity. This protein is a novel glutaminase possessing excellent thermostability and the like.

Thermostable glutaminase and thermostable glutaminase gene

Growing community of inventors

Ikoma, Japan

Naotake Ogasawara