National Institute of Advanced Industrial Science and Technology

Dna Chip Research Inc.

Naoshi Kojima

Yasuo Komatsu

Yoshio Kato

Kosuke Sato

Ken Nonaka

Yumi Fujinawa

Provided is a method for synthesizing a protein, into which a nucleobase amino acid (NBA) is introduced at a desired position, that comprises: a step for preparing mRNA into which a modified codon is inserted at a desired position downstream of an initiation codon; and a step for translating the aforesaid mRNA into a protein in the presence of tRNA, said tRNA recognizing the modified codon and being acylated with the NBA. Also provided is a ribozyme that catalyzes the aminoacylation of tRNA and comprises two RNA molecules.

Amino acid-modified nucleic acid and utilization thereof

Provided are a solid-phase carrier for solid-phase synthesis of nucleic acid having improved amino group reactivity, and an aminated oligonucleotide to which an amino group has been attached. The present invention relates to an aminated oligonucleotide represented by formula (I).

Solid-phase carrier for aminated oligonucleotide

An aminated oligonucleotide probe is provided, in which the amino group possesses improved reactivity. The present invention relates to an oligonucleotide probe, which is represented by general formula 1: (wherein Ris a hydrogen atom or a protecting group for an amino group, Rand Rare each independently a divalent organic group, and A is an oligonucleotide).

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Ibaraki, Japan

Naoshi Kojima