The University of Tokyo

Toyo Boseki Kabushiki Kaisha

Probex Inc.

Naomi Misawa

Kenji Masuda

Takeaki Ozawa

Shigeaki Nishii

Kenji Miura

Tasuku Okamoto

The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex.

Method for highly sensitive detection of protein-protein interaction

Growing community of inventors

Tokyo, Japan

Naomi Misawa