Mitsubishi Chemical Corporation

Mitsubishi Kasei Corporation

Naomi Kitamura

Takeshi Shimomura

Keiji Miyazawa

Toshiya Kawaguchi

Kazuhiro Takahashi

Kazunori Yamada

Yuuki Morimoto

Rie Matsui

Yutaka Teranishi

Shigetada Nakanishi

Hirohito Tsubouchi

Daiji Naka

Yoshiko Yoshiyama

Yasushi Daikuhara

Takehisa Ishii

Michiru Kamizono

Tatsurou Shibui

A novel protein having inhibitory activity on the protease activity of hepatocyte growth factor (HGF) activator was purified and isolated, and its molecular weight (ca. 40,000 daltons) and its N-terminal and partial amino acid sequences were determined. A gene coding for the protein was cloned, and the gene DNA was incorporated into a vector, for transforming host cells. Cultivation of the transformant gave the desired protein. The protein can be used as an in vivo or in vitro control factor for HGF or HGF activator. It is also useful as an antigen to be used in producing an antibody to be used as means for kinetic studies of the protein, or as a standard in assay systems therefor.

Protein, DNA coding for same and method of producing the protein

A novel protein having inhibitory activity on the protease activity of hepatocyte growth factor (HGF) activator was purified and isolated, and its molecular weight (ca. 30,000 daltons) and partial amino acid sequence were determined. A gene coding for the protein was cloned, and the gene DNA was incorporated into a vector, for transforming host cells. Cultivation of the transformant gave the desired protein. The protein can be used as an in vivo or in vitro control factor for HGF or HGF activator. It is also useful as an antigen to be used in producing an antibody to be used as means for kinetic studies of the protein, or as a standard in assay systems therefor.

A gene encoding a protease derived from human serum and having an activity to convert single-chain hepatocyte growth factor (HGF) into active two-chain HGF and a method of producing the protease by the use of said gene are provided. A method of producing a precursor protein of said protease is also provided.

Hepatocyte growth factor activating protease and gene encoding the

According to the present invention, there are provided hepatic parenchymal cell growth factor obtained by recombinant DNA technology, a gene coding for the factor, an expression vector capable of expressing the gene, a cell, in particular animal cell, transformed with the expression vector, and a process for producing the hepatic parenchymal cell growth factor.

Hepatic parenchymal cell growth factor gene encoding the same, process

Provided herein are a DNA fragment which contains a base sequence coding for a non-A non-B hepatitis-specific antigenic protein occurring in cells of the liver affected with non-A non-B hepatitis, an expression vector in which said DNA fragment is inserted into a cloning site present downstream from a promoter thereof, a transformant obtained by introducing said expression vector into a host, and a process for producing said antigenic protein which comprises providing said expression vector, transforming a host with said expression vector, culturing the transforming host and collecting the protein produced therein.

DNA fragments coding for antigens specific to non-A non-B hepatitis,

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Moriguchi, Japan

Naomi Kitamura