International Diagnostic Technology

Naomi Kameda

Fred H Deindoerfer

Gunner Bolz

Robert Wang

Charles R Gifford

Chun P Hu

An improved method for the fluorometric measurement of a fluorescent label on a solid support surface in which the surface is read while coated with a continuous aqueous layer. For reading in a horizontal position, the surface is coated by immersion into a contained aqueous solution, and removed and read prior to evaporation to discontinuity. For reading in a vertical position, a layer of humectant is deposited on the surface to retain the water content.

Method for reading a wet fluorescent surface

A method for quantitation of antigens or antibodies from a liquid sample on a solid surface. The sample is first sorbed directly to a nonimmunological surface. Then the surface is exposed to antibody or antigen labelled preferably with a fluorogen, to cause an immunological reaction to occur. After removal of unreacted reagent from the surface, the quantity of reacted labelled reagent is determined, as for example, fluorogen by a fluorometer. When the sample includes significant quantities of protein other than the antigen or antibody to be quantitated, buffer protein unreactive with the labelled antigen or antibody is added in concentrations sufficient to minimize the dependence of sorbed antigen or antibody upon variations in the concentration of such other protein.

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Foster City, CA, United States of America

Naomi Kameda