Hitachi, Ltd.

Hitachi High-Tech Corporation

Hitachi-Lg Data Storage, Inc.

Hitachi-High-Technologies Corporation

Naoko Senda

Kentaro Osawa

Daisuke Tomita

Ryota Nakajima

Hideyuki Noda

Kazumichi Imai

Hiroaki Nakagawa

Toshinari Sakurai

Takayuki Nozaki

Yoko Inoue

Masaharu Kiyama

Guangbin Zhou

Chihiro Manri

Toyoshige Kobayashi

Akihiro Shimase

Shizu Takeda

Shizu Matsuoka

Nozaki Takayuki

Provided is a method for detecting a microorganism in a test sample containing cells, including a step of adding the sample to a microorganism culture medium, a step of culturing the microorganism culture medium containing the sample, a step of sampling a part of the culture medium at a predetermined time, a step of acquiring the ATP level of the sampled culture medium, and a step of detecting the microorganism in the sample based on the change of the ATP level over time.

Microorganism test

The present invention provides a method and an apparatus for analyzing a cell state, cell death in particular, in an interior of a spheroid non-invasively and quantitatively when the spheroid is cultured. More specifically, the present invention provides a method and an apparatus for analyzing a cell state by implementing optical imaging of a spheroid by using an optical instrument characterized by a high resolution and analyzing the internal structure of the spheroid.

Method for analyzing state of cells in spheroid

Provided is a method for non-invasively and quantitatively determining multilayerization and differentiation when culturing a cell sheet. Provided is a method for determining a cell state by imaging a cell sheet by using an optical instrument characterized by having a high resolution, and then analyzing the inner structure thereof.

Cell culture device and image analysis device

Provided is a cell culturing vessel for holding and culturing cells. It has a first vessel that stores a culture medium and cells or only a culture medium, a second vessel that is placed above the first vessel and stores a culture medium and cells or only a culture medium, a main vessel that holds the first vessel and houses the second vessel, and a lid member that engages with the main vessel. The main vessel has a pressing member that fixes and holds the first vessel in the main vessel and the second vessel is eccentrically held in the first vessel by the pressing member.

Cell-culturing vessel and cell-culturing device using same

There is provided a method for noninvasively evaluating the cell state (proliferation, multi-layering, and differentiation) of a cell sheet as a mimic tissue at the time of culturing the cell sheet. The method is characterized in that an analysis of an amino acid is conducted with the use of the culture supernatant of a cell sheet to monitor a change in the concentration of any amino acid selected from a group of 5-species of amino acids (Ile, Val, Ser, Leu, and Ala), thereby making a determination.

Method for determining cell state and autoanalyzer using said method

An optical measurement apparatus has a light source that emits a laser beam that is branched into signal light and reference light. An objective lens condenses the signal light on a measurement target to cause the measurement target to be irradiated; and a condensing position of the signal light is scanned in an optical axis direction. An interference optical system combines the signal light reflected or scattered from the measurement target with the reference light, and generates a plurality of interfering light beams having phase relationships different from one another that are detected by photodetectors. The detection signals are output as electrical signals; and a signal processing unit performs a predetermined arithmetic operation on the plurality of detection signals. The signal processing unit subtracts reflection light components from a predetermined portion of the measurement target from the plurality of detection signals or signals generated using the detection signals.

Optical tomography observation apparatus

A clear image of a measurement target in optical coherence tomography (OCT) is obtained while suppressing influence of reflected light from a specific portion. Included are a laser beam source, a beam splitter that splits a laser beam into a signal beam and reference beam; an objective lens that focuses the signal beam onto a measurement target in a container, a unit that moves the signal beam focus position, an objective lens that focuses the reference beam, a reflecting mirror, a flat plate arranged between the objective lens and reflecting mirror, and interference optics that combine the signal beam reflected by the measurement target with the reference beam reflected by the reflecting mirror and having passed through the objective lens. Three or more interference beams with different phases, and photodetectors that detect the interference beams are generated, and two of the objective lenses are the same lenses.

Optical image measuring apparatus

Growing community of inventors

Tokyo, Japan

Naoko Senda