Suntory Holdings Limited

Naoko Okitsu

Yoshikazu Tanaka

Keisuke Matsui

Kazunari Iwaki

Tadayoshi Hirai

Katsuro Miyagawa

Saori Takeyama

Yukihisa Katsumoto

Noriko Nakamura

Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.

Buckwheat-derived C-glycosyltransferase gene and utilization thereof

The present invention provides a high rebaudioside M content non-genetically modifiedplant comprising rebaudioside M at higher content as compared with the wild typespecies. The present invention also provides a method of producing such a high rebaudioside M content non-genetically modifiedplant, and a dry leaf obtainable from such a plant.

Rebaudioside M-rich stevia plant

The present invention provides a high sweet contentplant having a variation at a portion corresponding to SEQ ID NO: 1, a method of producing or screening for the same, etc.

High-sweetening-content stevia plant and method for screening same

Provided is a polynucleotide encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4'-glucoside, wherein the polynucleotide is selected from the group consisting of: (a) a polynucleotides comprising the base sequence of SEQ ID NO: 1 or SEQ ID NO: 5; (b) a polynucleotide that hybridizes, under stringent conditions, with a polynucleotide comprising the base sequence complementary to the base sequence of SEQ ID NO: 1 or SEQ ID NO: 5; (c) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 6; (d) a polynucleotide encoding a protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 6.

Glycosyltransferase gene and use thereof

Provided is a polynucleotide encoding a protein having activity that transfers a sugar to the hydroxyl group at the 4'-position of a flavone, wherein the polynucleotide is selected from the group consisting of: (a) a polynucleotide composed of the base sequence of SEQ ID NO: 1; (b) a polynucleotide that hybridizes under stringent conditions with a polynucleotide composed of a base sequence complementary to the base sequence of SEQ ID NO: 1; (c) a polynucleotide encoding a protein composed of the amino acid sequence of SEQ ID NO: 2; and, (d) a polynucleotide encoding a protein composed of an amino acid sequence in which one or a plurality of amino acids have been deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:2.

Provided is a polynucleotide encoding a protein having an activity to transfer a sugar to the hydroxy groups at the 4'- and 7-positions of a flavone. The polynucleotide is selected from the group consisting of: (a) a polynucleotide which comprises a base sequence represented by SEQ ID NO: 1, 3, or 12; (b) a polynucleotide which hybridizes to a polynucleotide comprising a base sequence complementary to a base sequence represented by SEQ ID NO: 1, 3, or 12 under high stringency conditions, and encodes a protein having an activity to transfer a sugar to the hydroxy groups at the 4&#x2032;- and 7-positions of a flavone; (c) a polynucleotide which encodes a protein comprising an amino acid sequence represented by SEQ ID NO: 2, 4, or 13; (d) a polynucleotide which encodes a protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in an amino acid sequence represented by SEQ ID NO: 2, 4, or 13 and having an activity to transfer a sugar to the hydroxy groups at the 4&#x2032;- and 7-positions of a flavone; etc.

Growing community of inventors

Osaka, Japan

Naoko Okitsu