Kabushiki Kaisha Toshiba

Sekisui Medical Co., Ltd.

Naoko Nakamura

Koji Hashimoto

Keiko Ito

Nobuhiro Gemma

Osamu Sato

Masayoshi Takahashi

Tsuneaki Kudou

Michie Hashimoto

One embodiment is related to a method of analyzing plural samples. The method includes amplifying a plurality of samples using a first primer and second primer, wherein the first primer includes a tag sequence having a sequence different from a sample to one another and wherein a second primer used in pair with the first primer in independent reaction systems for the respective samples to obtain an amplified product in which the tag sequence is introduced, mixing amplified products obtained in the plurality of reaction systems, making the mixed amplified product react with a nucleic acid probe immobilized on a substrate, and detecting the amount of hybridization that has occurred.

Sample analysis method and assay kit used therein

Kits for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5' terminal side and B3, B2and B1regions in this order from a 3&#x2032; terminal side, and additionally an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2to B1regions in the target nucleic acid, determining the respective regions in such a manner that the FP and F2 regions and/or the BPc and B2regions have an unoverlapping region of at least 10 bases or more and overlapping regions of 10 bases or less, and designing the primers according to the regions.

Assay kit for use in method of detecting a target nucleic acid

A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.

Method for detecting a target nucleic acid sequence

There is provided a nucleotide primer set for LAMP amplification, used for detecting genotypes of single-nucleotide polymorphisms G590A, G857A and T341C of a NAT2 gene. There is also provided a nucleotide probe for detection of an amplification product amplified with the primer set according to the present invention. There is also provided a method of detecting the genotypes of NAT2 gene single-nucleotide polymorphisms G590A, G857A and T341C by using the primer set according to the present invention.

Nucleotide primer set and nucleotide probe for detecting genotype of N-acetyltransferase-2 (NAT2)

Provided is a nucleic acid primer for LAMP amplification for use in the detection of human papilloma virus and identification of its genotype. The present invention also provides a method of detecting human papilloma virus and identifying its genotype, includes a step of amplifying the nucleic acid chains in a sample in LAMP reaction by using multiple primers including at least one primer selected from the nucleic acid primers according to the present invention and a step of detecting presence of amplified products after the amplification reaction and identifying their genotypes.

Method of detecting human papilloma virus by using nucleic acid amplification method and nucleic acid chain-immobilized carrier

A method of designing primers for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5' terminal side and Bc, B2and B1regions in this order from a 3&#x2032; terminal side, and additionally an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2to B1regions in the target nucleic acid, determining the respective regions in such a manner that the FP and F2 regions and/or the BPc and B2regions have an unoverlapping region of at least 10 bases or more and overlapping regions of 10 bases or less, and designing the primers according to the regions.

Method of detecting amplification product or target nucleic acid

There is provided is a nucleotide primer set for LAMP amplification used for detecting genotypes of single-nucleotide polymorphisms C677T and A1298C of an MTHFR gene. There is also provided a nucleotide probe for detecting an amplification product amplified by the primer set according to the present invention. There is also provided a method of detecting the genotypes of the single-nucleotide polymorphisms C677T and A1298C in the MTHFR gene, by using the primer set according to the present invention.

Nucleotide primer set and nucleotide probe for detecting genotype of methylene tetrahydrofolate reductase (MTHFR)

An improved flip-flop with a scan path comprises a front page circuit driven in response to a clock signal and multiplexers for receiving a data signal and a scan test signal. Each of the multiplexers comprises a single stage of FETs connected in series between a power source and a ground. This arrangement can remarkably improve an operation frequency.

Growing community of inventors

Kawasaki, Japan

Naoko Nakamura