Takeda Pharmaceutical Company Limited

San-ei Chemical Industries, Ltd.

Naoko Fujimoto

Akitsu Hotta

Hiroki Iida

Rumiko Ochiai

Saburo Kohara

Masataka Ifuku

Yukimasa Makita

Kumiko Iwabuchi

Shiro Oohashi

Eriya Kenjo

The present invention provides a delivery technique for delivering a gene modification tool capable of providing a high gene modification efficiency in cells. The composition according to the present invention is a composition for inducing gene modification at a target gene locus in a cell, the composition containing 1) a compound represented by formula (I) or a salt thereof; 2) a structural lipid; and 3) a guide RNA or a DNA including a sequence encoding the guide RNA, and/or an RNA-guided nuclease or a nucleic acid including a sequence encoding the RNA-guided nuclease. In formula (I), n represents an integer of 2 to 5, R represents a linear Calkyl group, a linear Calkenyl group, or a linear Calkadienyl group, and wavy lines each independently represent a cis-type bond or a trans-type bond.

Composition for modifying target gene

Process for preparing a layered hydrogel product is provided by separately or simultaneously pouring into a mold of a desired shape an aqueous solution (I) containing at least one gelling agent, and an aqueous solution (II) containing xanthan gum and at least one thickening agent including locust bean gum, tara gum, cassia gum and glucomannan, and cooling the resulting material to give a layered hydrogen product.

Process for preparing a layered hydrogel product

Growing community of inventors

Kyoto, Japan

Naoko Fujimoto