Japan Science and Technology Agency

Naoko Abe

Hiroshi Abe

Yasuaki Kimura

Hiroki Murase

Kosuke Nakamoto

A method for producing a capped RNA which is an RNA having the 5′-end modified with a cap, the method including: reacting an activated capping compound represented by

Method for producing capped RNA

A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, Rrepresents a decomposable protecting group, and Rrepresents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3'-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting Rand forming a sticky end with a 3′-protruding end.

Primer, device for producing double-stranded DNA using primer, and method for producing double-stranded DNA using primer

where Arepresents —S—, —S—S—, or —Se—, B represents a base, and Rrepresents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3'-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3′ ends blunt; and a photoirradiation unit for deprotecting Rand forming a sticky end with a 3′-protruding end.

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Nagoya, Japan

Naoko Abe