Eisai Company, Limited

Shimadzu Corporation

Eisai R&d Management Co., Ltd.

Other

Naoki Asakawa

Kaoru Murata

Nariyasu Mano

Yutaka Yoshida

Tadashi Sato

Yoshiya Oda

Yasuo Miyake

Yosuke Iwata

Yasuo Ishibashi

Yasushi Ishihama

Fumio Kakimoto

Yuki Sato

Hachiro Sugimoto

Sumio Watanabe

Masahiro Yonaga

Taro Terauchi

Takashi Doko

Masayuki Tanaka

Masahiro Kawahara

Naoki Asai

Yukio Nishizawa

Tadayuki Yamaguchi

Naoya Kishi

Katsuaki Kaito

Toshinobu Miwa

Eiichi Yamamoto

Hiroshi Ohe

Kosuke Fukuzawa

Yukuo Nezu

Yuichiro Hayashi

Yumi Yokoyama

Akinori Akaike

Motoaki Mori

Motoaki Murakoshi

A liquid delivery device includes a liquid delivery pump that sucks a mobile phase, which is liquid, from a mobile phase container containing the mobile phase through a suction pipe whose end is immersed in the mobile phase in the mobile phase container, and feeds the mobile phase, and at least one dissolved substance removal filter that is provided at a position upstream of the liquid delivery pump on a path through which the mobile phase flows and is filled with a filler having the property of adsorbing a dissolved substance in the mobile phase.

Liquid delivery device and liquid chromatograph

An elution test method measures the elution process of a specific component in a process where a preparation containing at least the specific component subject to a change with time in chemical properties after elution and an impurity component not subject to a change with time in chemical properties after elution is eluted into a test liquid. The method obtains a ratio of absorbance k of the impurity component and a ratio of absorbance k' of the specific component at two isosbestic point wavelengths &#x3bb;in a spectrum including the specific component and its decomposed matter, and calculates at least one of the absorbances A(t), A(t) of said specific component by using k&#xd7;C(t)&#x2212;C(t)=k&#xd7;A(t)&#x2212;A(t) and A(t)/A(t)=k&#x2032; from the absorbance C(t), C(t) at said two isosbestic point wavelengths &#x3bb;measured at the plural time points t in the elution process of said preparation, and converts the result to an elution concentration.

Elution test method and apparatus

Device for improving detection sensitivity in low flow velocity high performance liquid chromatographic apparatus. Preferredly, diffusion promoting device connected at just before separation column and having function of improving detection sensitivity for use in low flow velocity high performance liquid chromatographic apparatus. Also, method for improving detection sensitivity in low flow velocity high performance liquid chromatographic apparatus.

Diffusion promoting device for low flow velocity gradient high performance liquid chromatography

The present invention provides a novel compound exhibiting an excellent suppressing effect to cell injury caused by radicals and neurotoxicity induced by excitatory nuerotransmitters such as glutamate. Specifically, it provides a compound represented by the following formula, a salt thereof or a hydrate of them. Wherein Z represents a bivalent organic group and the like of from 2 to 3 carbon atom(s) which may have a substituent; and Rrepresents a carboxyl group and the like.

Atisane compounds and use thereof

The present invention provides a packing for chromatography. Specifically, it provides a packing for chromatography in which a hydrophilic polymeric material is bound to the surface of a porous carrier, and a carbon chain is chemically bound inside the pore. By the use of the packing according to the present invention for analysis of low molecular weight compounds in a biological sample, a pretreatment such as removal of proteins etc. becomes unnecessary, and the biological sample can be directly analyzed.

Column packaging for chromatography

The present invention provides a device for improving detection sensitivity and a method for improving the detection sensitivity for use in a low flow velocity high performance liquid chromatographic apparatus. That is, the present invention is a diffusion promoting device connected at just before a separation column and having a function for improving detection sensitivity for use in a low flow velocity high performance liquid chromatographic apparatus.

Diffusion promoting apparatus for low flow velocity gradient high-speed liquid chromatography

The present invention provides a device capable of simply formulating an arbitrary gradient pattern in a low flow gradient high performance liquid chromatography. That is, the present invention provides a split tubing apparatus for a gradient high performance liquid chromatography, which comprises a solvent inlet part, a split tubing part split into two or more plural tubes, and a solvent outlet part.

Splitted tubing apparatus for gradient high performance liquid chromatography

A high-performance liquid chromatography apparatus and a process for conversion of mobile phase, enabling a trace amount of sample for NMR analysis to be efficiently separated and prepared, includes steps of separating a target ingredient from the sample by the high-performance liquid chromatography, trapping the target ingredient in a trapping column using a different mobile phase, replacing water by deuterium oxide and eluting this target ingredient from the trapping column using deuterated solvent other than deuterium oxide.

HPLC apparatus for fractioning and preparing sample for NMR spectrometry and method of changing mobile phase

A sample solution is sent into a concentration column through a three-way joint and a three-way switching valve to trap and concentrate the sample components on the concentration column, followed by injecting the sample solution into an injector by operating a first dilution solvent pump. When a large volume of the sample solution is to be sent or when the sample solution has a high solvent strength, trapping and concentration on the concentration column may be facilitated by replacing the sample solvent with a second dilution solvent by allowing the second dilution solvent to flow into the three-way joint after setting the flow rate of the second dilution solvent from a second dilution solvent vessel to a prescribed flow rate using a second dilution solvent pump.

Liquid chromatograph

Separating agent comprising bonded conalbumin

A packing for chromatography whereby a low molecular weight substance contained in biological samples can be analyzed directly at a high accuracy without any pretreatments. This packing is combining a protein such as avidin and ovomucoid to the support via a straight or branched spacer optionally having a functional group such as a cyano group. Enantiomers interact differentially with proteins, and thereby optically separated. Further, a purification column packed with said packing is disclosed. According to the present invention, a low molecular weight substance can be directly and accurately analyzed without requiring any pretreatment for removing biological macromolecules. When it is employed in the purification column, the time required for the pretreatment can be shortened.

Packings combining protein to a support via a spacer

An injection composition comprises a pharmacologically effective amount of a modified tPA, 47.5 mM or more of an amino acid or a salt thereof and is improved in view of stability and solubility.

Modified tPA-containing injection composition having increased solubility

The components present in the sample with a mobile phase A in 1st section is separated. In 2nd section there is provided with an inserting line of a dilute solution for diluting the mobile phase A and a trapping column which forces the dilute solution to pass but catches the components by connecting said line to said column. In 3rd section, the components caught by the trapping column are introduced into mass spectrometer by using a mobile phase B. It is preferable to use respectively different mobile phases for the mobile phase A and the mobile phase B. It is good to install separating columns in 1st and 3rd sections. It is also desired to add the retaining apparatus of the components separated in 1st section.

Exchange method of mobile phase in high-performance liquid

A medical composition which contains a tissue Plasminogen Activator (tPA) in combination with meglumine or a salt thereof.

tPA-containing medical composition

Growing community of inventors

Ibaraki, Japan

Naoki Asakawa