Ajinomoto Co., Ltd.

Naoki Akiyoshi

Osamu Kurahashi

Kazuhiko Matsui

Tsuyoshi Nakamatsu

Shinichi Sugimoto

Yoshio Kawahara

Eiichiro Kimura

Seiko Hirano

Gen Nonaka

Tsuyoshi Osumi

Yumi Matsuzaki

Kanae Nakamura

Masakazu Sato

Yoshihiko Hara

Hiroshi Izui

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from, preferably an enzyme that functions at a higher temperature compared with that of. PCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived frompreferably an enzyme that functions at a higher temperature compared with that ofPCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

Thermophilic amino acid biosynthesis system enzyme gene of thermotolerant coryneform bacterium

A method for producing L-glutamic acid by fermentation, which comprises culturing a microorganism having L-glutamic acid-producing ability in a medium having pH of 5.0 or less, wherein a total content of an organic acid that inhibits growth of the microorganism at the pH is one at which the growth of the microorganism is not inhibited; and a method for producing L-glutamic acid by fermentation, which comprises culturing a microorganism having L-glutamic acid-producing ability at a first pH at which growth of the microorganism is not inhibited by an organic acid in a medium, and then culturing the microorganism at a second pH that is suitable for L-glutamic acid production by the microorganism and is lower than the first pH.

Method for producing L-glutamic acid

A method for producing L-glutamic acid by fermentation, which comprises culturing a microorganism having L-glutamic acid-producing ability at a first pH that is suitable for growth of the microorganism, and then culturing the microorganism at a second pH that is suitable for L-glutamic acid production by the microorganism and is lower than the first pH.

Growing community of inventors

Kawasaki, Japan

Naoki Akiyoshi