Emd Millipore Corporation

Cabot Corporation

Millipore Corporation

Merck Patent Gmbh

Upfront Chromatography A/s

Nanying Bian

Neil P Soice

Chen Wang

Joaquin A Umana

Shari Spector

Matthew T Stone

Kwok-Shun Cheng

Collin P Galloway

Joseph E Johnson

Senthilkumar Ramaswamy

Brian Gagnon

Joe Orlando

Robert Smith

Santosh Rahane

Mikhail Kozlov

John Charkoudian

Martin Siwak

Christopher Gillespie

Dennis Aquino

Jie Chen

Andrew Lyddiatt

Melissa Holstein

Yuan Wong

Senthil Ramaswamy

Kara Levine

John Dana Hubbard

Daniel Scheid

Umana Joaquin

Matthew Turiano

Lyddiatt Andrew

Sapna Mehtani

Chia-Yun Sun

Kristen Cotoni

Senthikumar Ramaswamy

Embodiments described herein relate to novel chromatography media for removing anti-A and/or anti-B antibodies from a sample, as well as methods of using the same. The media described herein have several advantages over previously described media including, acid and alkaline stability.

Affinity chromatography media for removal of anti-a and/or anti-b antibodies

The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

Methods of reducing level of one or more impurities in a sample during protein purification

The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.

Caustic stable chromatography ligands

The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of, such that the column can be cleaned using both acidic and alkaline solutions.

Methods of cleaning a protein A based affinity chromatography column

Embodiments described herein relate to methods of evaluating quality of a chromatography media for removal of anti-A or anti-B antibodies from a sample, where the methods employ use of purified monoclonal IgM-A and IgM-B antibodies.

Methods of evaluating quality of a chromatography media which binds anti-A or anti-B antibodies

Embodiments described herein relate to methods of evaluating quality of a chromatography media for removal of anti-A or anti-B antibodies from a sample, where the methods employ use of purified Lectins.

Methods of evaluating quality of media suitable for removing anti-A or anti-B antibodies

The present invention provides methods for increasing purity of an Fc-containing protein by removing protein aggregates during the Protein A chromatography step used during the purification of the Fc-containing protein.

Methods of increasing protein purity using protein A based chromatography

The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely effecting the yield of the desired protein product.

The present invention relates to new separation materials with improved binding capacity, its manufacturing, and application, especially for binding protein A.

Surface modification of porous base supports

The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as,protein A, as well as methods of making and using such ligands.

The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

Media for affinity chromatography

The present invention relates to chromatography matrices including ligands based on one or more domains of immunoglobulin-binding proteins such as,Protein A (SpA), as well as methods of using the same.

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The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

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Lexington, MA, United States of America

Nanying Bian