Monsanto Technology LLC

Nancy M Houmard

Shihshieh Huang

Larry A Gilbertson

Edwards M Allen

James Kevin Roberts

Thomas M Malvar

Sergey Ivashuta

Paul S Chomet

Hongwu Jia

Michael R Kerns

Mark E Leibman

David K Kovalic

Emil M Orozco, Jr

Yuanji Zhang

Elysia K Krieger

Linda L Lutfiyya

Liang Guo

Michael H Luethy

Albert P Kausch

Sara Elizabeth Heisel

James K Roberts

Philip W Miller

Lucille B Laccetti

Alessandra Frizzi

Albert Kausch

Emil M Orozco

Edwards Allen

Sergey I Ivashuta

Sara E Heisel

David E Kovalic

Yaunji Zhang

Michael H Leuthy

The present invention is in the field of plant breeding and drought tolerance. More specifically, the invention includes a method for breeding corn plants containing one or more markers that are associated with tolerance to low water conditions. Markers provided herein include markers genetically linked to proteins comprising cold shock domains, such as the CSD1 and CSD2 proteins. The invention further includes germplasm and the use of germplasm containing at least one marker associated with drought tolerance for introgression into elite germplasm in a breeding program, thus producing drought resistant germplasm. Other embodiments of this invention include transgenic drought tolerant corn plants and corn plant cells, and methods of detecting drought tolerance genes and chromosome regions associated with drought tolerance in a plant genome.

Methods of creating drought tolerant corn plants using markers linked to cold shock domain-containing proteins and compositions thereof

This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

Methods for producing hybrid seed

The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

Recombinant DNA constructs and methods for controlling gene expression

Engineered miRNA precursors for suppressing target gene expression in plants

The current invention provides the promoter of thenuclear gene encoding chloroplast-localized fructose-1,6-bisphosphate (F16BP) aldolase. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the chloroplastic F16BP aldolase promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize chloroplast aldolase promoter compositions and methods for use thereof

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North Stonington, CT, United States of America

Nancy M Houmard