Beckman Coulter, Inc.

Coulter International Corp.

Nancy J Torke

Lidice L Lopez

John A Maples

Diana B Careaga

Mark A Wells

Carlos A Perez

Cheng Qian

Ziling Huo

Methods are provided for measurement of nucleated red blood cells in a blood sample. The methods include exposing a blood cell sample to a reagent system to lyse mature red blood cells, subsequently analyzing nucleated red blood cells in a flow cell by axial light loss, DC impedance, and medium angle light scatter measurements; by axial light loss, low angle light scatter, and medium angle light scatter measurements; or by axial light loss, DC impedance, low angle light scatter, and medium angle light scatter measurements; and then differentiating nucleated red blood cells from other cell types by using measured signals and/or functions thereof.

Method of measurement of nucleated red blood cells

A composition for enhancing differential staining of RNA, DNA and granules in a sample comprising cells contains a first fluorescent dye that can bind specific binding sites and non-specific binding sites in the sample. This first dye emits fluorescence at a first wavelength. The composition contains at least an additional component, which is a second non-intercalating dye in the composition that competes with said first dye for binding to the nonspecific binding sites, or a permeabilizing agent to enhance permabilization of the dyes into the cells, or both. The molar ratio of the second dye and the first dye is at least about 20:1.

Dye compositions which provide enhanced differential fluorescence and light scatter characteristics

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Miami, FL, United States of America

Nancy J Torke