Eastman-kodak Company

Cetus Corporation

Nancy F Green

Thomas J Cummins

Richard C Sutton

Fred T Oakes

Sheryl S Sullivan

Randall D Madsen

There are described a device and method for doing confined reactions such as PCR amplification and detection, wherein solids (e.g., beads) used to obtain separation between bound and 'free' label reagents, are transferred from region to region, specifically through a wash liquid so as to wash off the 'free' label reagent from the solids. Separate chambers have dividers that are overcome by piercing or by liquification, to create passageways for the transfer of the solids. The passageways remain contained within the device.

Device for moving a target-bearing solid through a liquid for detection

Herpes simplex viral antigen can be readily determined by contacting a specimen containing Herpes simplex virus of herpes simplex viral-infected cells with polymeric particles which have a surface area of from about 0.1 to about 600 m.sup.2 /g. Within a few minutes of this contact, antigen which is bound to the particles is contacted with antibodies thereto so as to form an immunological complex on the particles. Bound complex is separated from uncomplexed materials, and the presence of the complex is then appropriately determined. A kit for determining herpes comprises the particles described above, a disposable test device having a microporous membrane and antibodies to herpes simplex viral antigen.

Method and kit for determination of herpes simplex viral antigen by

An extraction composition has been found useful for extracting antigen from herpes simplex virus. This composition has a pH of from about 8.5 to about 12, and comprises an alcoholamine or salt thereof, a nonionic surfactant comprised of a condensation product of an alkylphenol and ethylene oxide, cholic acid or a salt or derivative thereof and an anionic surfactant. Extraction of antigen is accomplished by contacting the extraction composition with a specimen suspected of containing herpes organisms under suitable conditions. Extracted antigen can be determined by forming an immunological complex with antibodies thereto and by detecting that complex. The extraction composition can be supplied as part of a diagnostic test kit.

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Pittsford, NY, United States of America

Nancy F Green