Chinese Academy of Agricultural Sciences

Beijing Foreland Biopharma Co., Ltd.

Nan Zheng

Songli Li

Jiaqi Wang

Shengguo Zhao

Jing Guo

Xingmin Zhang

Lei Xing

Ensi Wang

Zhuolin Dai

Liping Ji

Zhenfang Wang

Tie Liang

Xu Zhou

Shengxiu Niu

Yangdong Zhang

In a method for extracting microbial DNA from milk, the extracted DNA can be directly used for PCR amplification. The method includes the following steps: treating milk with TE solution, fully disrupting microbial cells by bead milling, immobilizing proteins in the middle layer between the aqueous and organic phases with high-vacuum silicone grease so as to prevent impurities in the middle layer from entering the aqueous phase, etc. The extracted DNA has a high degree of intactness, high concentration and purity.

Method for extracting total microbial DNA from milk

In a method for enriching and separating an anaerobic fiber-degrading bacterium on the basis of cellulosic magnetic nanoparticles, the cellulosic magnetic nanoparticles are prepared by coating cellulose on the surface of a magnetic nanomaterial. Bacteria attach to the cellulosic magnetic nanoparticles. Anaerobic fiber-degrading bacteria degrade the cellulose and then detach from the cellulosic magnetic nanoparticles, and the anaerobic fiber-degrading bacteria are obtained by means of separation using a magnet. The magnetic nano-separation technology can be used as a new technology to efficiently separate anaerobic fiber-degrading bacteria.

Method for enriching and separating anaerobic fiber-degrading bacterium on the basis of cellulosic magnetic nanoparticles

The present invention provides a heterocyclic group contained amino-methanol derivative, and salt, a preparation method and use thereof, and belongs to the medical field. The heterocyclic group contained amino-methanol derivative and the salt thereof of the present invention are used for preparing medicines for immune suppression and for the treatment of organ transplant rejection, or medicines for treating immune mediated inflammatory diseases, such as multiple sclerosis, systemic lupus erythematosus and rheumatoid arthritis.

Heterocyclic group contained amino-methanol derivative, and salt, synthetic method and use thereof

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Beijing, China

Nan Zheng