New England Biolabs, Inc.

Nan Dai

Ivan R Correa, Jr

Yu Brian Zheng

Zhenyu Zhu

Shengxi Guan

Aine Quimby

Richard J Roberts

Romualdas Vaisvila

Lana Saleh

Janine Borgaro

Megumu Mabuchi

June Pais

Laurence Ettwiller

Sriharsa Pradhan

George Tzertzinis

Ira Schildkraut

Jurate Bitinaite

Hang-Gyeong Chin

Bo Yan

Madalee G Wulf

Erbay Yigit

Sebastian Grünberg

Eric Wolf

The present disclosure relates, according to some embodiments, to compositions and analysis of RNA (e.g., dephosphorylated oligoribonucleotides) including, for example, natural and/or synthetic RNAs. A composition may comprise, for example, an endoribonuclease having an amino acid sequence that (i) corresponds to an amino acid sequence of a first species (e.g.,, and) or (ii) is a non-naturally occurring sequence; and/or an RNA end repair enzyme having an amino acid sequence that (i) corresponds to an amino acid sequence of a species other than the first species (e.g., a bacterial species or a bacteriophage species) or (ii) is a non-naturally occurring sequence.

Compositions and analysis of dephosphorylated oligoribonucleotides

Provided herein is a method for making an cDNA library, comprising adding an affinity tag-labeled GMP to the 5' end of targeted RNA species in a sample by optionally decapping followed by incubating the sample with an affinity tag-labeled GTP and a capping enzyme, enriching for RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag, reverse transcribing the enriched RNA to produce a population of cDNAs, and adding a tail to the 3&#x2032; end of the population of cDNAs using a terminal transferase, to produce an cDNA library.

Enrichment and sequencing of RNA species

Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.

Mapping cytosine modifications

5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.

Compositions and methods for oxygenation of nucleic acids containing 5-methylpyrimidine

Nucleic acids comprising &#x3b2;-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described.

Compositions and methods for the transfer of a hexosamine to a modified nucleotide in a nucleic acid

Composition and methods of oxygenation of nucleic acids containing 5-methylpyrimidine

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Gloucester, MA, United States of America

Nan Dai