Nisshinbo Industries, Inc.

Nisshingo Industries, Inc.

Namiko Shiohata

Osamu Suzuki

Gen Masuda

Kazuko Matsumoto

Yoko Yoshikawa

Tatsuo Ichihara

Yoshiyuki Matsumura

A novel fluorescent group-containing carbodiimide compound having at least one group selected from a carboxyl group, a sulfo group, a phosphono group and a phospho group which have substitution of an alkali metal, an alkaline earth metal or a basic group containing a nitrogen or phosphorus atom, which is prepared by using a novel fluorescent group-containing carbodiimide compound precursor having a halogen atom or a sulfonic acid group; and a method for detecting a nucleic acid by hybridization utilizing a nucleic acid labeled with a labeling substance, which utilizes the fluorescent group-containing carbodiimide compound as the labeling substance.

Fluorescent group-containing carbodiimide compound precursor, fluorescent group-containing carbodiimide compound and methods for producing them

Method of detecting a nucleic acid using a fluorescent group-containing carbodiimide as a label

A nucleic acid-immobilized substrate which comprises a carrier comprising a base material and a compound having a carbodiimide group or an isocyanate group carried by the base material, and the same kind or different kinds of nucleic acids immobilized in the form of dots through the carbodiimide group or the isocyanate group at a plurality of sites on the carrier.

Nucleic acid-immobilized substrate

A novel fluorescent group-containing carbodiimide compound having at least one group selected from a carboxyl group, a sulfo group, a phosphono group and a phospho group which have substitution of an alkali metal, an alkaline earth metal or a basic group containing a nitrogen or phosphorus atom, which is prepared by using a novel fluorescent group-containing carbodiimide compound precursor having a halogen atom or a sulfonic acid group.

By immobilizing identical or different nucleic acids in a plurality of dot-like areas on a carrier which comprises a base material and compound carried on the base material, the compound having one or more alkylating groups, through intermediary of the alkylating group, a nucleic acid-immobilized substrate on which nucleic acids are firmly immobilized in fine dot area without reference to chain length of nucleic acids is provided, which enables efficiently introducing the nucleic acids onto the base material in a simple manner and can be produced with a simple apparatus.

Using the chemiluminescent group-containing carbodiimide compound represented by the following formula or its quaternary ammonium salt as the label in the nucleic acid detection method or immunoassay, labeling can be made efficiently for a short time, a nucleic acid derived from nature can be labelled, and highly sensitive assay is enabled. ##STR1##

Chemiluminescent group-containing carbodiimide compound

Using the fluorescent group-containing carbodiimide compound represented-by the following formula (I) as the label and the like in the nucleic acid detection method, immunoassay, or chemiluminescence assay, labeling can be made efficiently for a short time, a nucleic acid derived from nature can be labelled, and highly sensitive assay is enabled.

Fluorescent group-containing carbodiimide compound

As a novel digoxigenin derivative which makes the labeling procedure simple, can be used to label a naturally-occurring nucleic acid, and enables highly sensitive assay, carbodiimide group-containing digoxigenin derivative represented by the following formula is used as the label in a nucleic acid detection method or immunoassay. ##STR1##

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Chiba, Japan

Namiko Shiohata