The Rockefeller University

Temasek Life Sciences Laboratory Limited

Michigan State University

Gendaq Limited

Other

The Rockfeller University

Institute of Molecular Agrobiology

Pioneer Hi-bred International, Inc.

Plastid As

Webber, Philip Michael

Nam-Hai Chua

Jianru Zuo

Qi-Wen Niu

Giovanna Frugis

Willem F Broekaert

Natasha V Raikhel

Anil Kush

Yen Choo

Philip N Benfey

Christopher Graeme Ullman

Eric Lam

Juan Pablo Sanchez

Jian Ye

Jing Qu

Fumiaki Katagiri

Qiwen Niu

Qi Xie

Hiroharu Banno

Takashi Aoyama

William James Gordon-Kamm

Kenji Oeda

Lianghui Ji

Lin Cai

Alexander Emelyanov

Sergey Parinov

Fumiharu Ishige

Srinivasan Ramachandran

Mi Jung Kim

Xiuren Zhang

Simon Geir Moller

Shih-Shun Lin

Yoshihisa Ikeda

Shi-Qiang Gao

Hans Erik Molager Christensen

Philip M Gilmartin

Alexander Van Der Krol

Seong Wook Yang

Jose L Reyes Taboada

Yuxin Hu

Takashi Soyano

Jodi Maple

Jose Reyes Toboda

The present invention relates to the field of plant molecular biology, more particularly cotton WRINKLED 1-like (WREL) genes. More specifically, the present invention relates to cotton WRIL genes whose products act as transcription factors of genes involved in fatty acid biosynthesis. The present invention also relates to methods of increasing cotton fiber length in cotton. In one embodiment, the methods involve modulating the level of activity of an enzyme involved in a fatty acid biosynthesis in the host cotton cell and/or culturing the host cotton cell. In another embodiment, the methods involve the manipulation of transcription factors which can regulate an enzyme involved in fatty acid biosynthesis.

Methods for increasing cotton fiber length

The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.

Control of pests in plants

The present invention relates to the field of plant molecular biology and gene silencing. More particularly, the present invention relates to gene silencing of Sugar-dependent 1 (JcSDP1) in. JcSDP1 encodes a patatin-domain triacylglyerol lipase. Silencing of JcSDP1 enhances seed oil accumulation in

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The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for gene silencing or RNA down regulation, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence or to down regulate RNA.

MicroRNAs

The present invention relates generally to chemical-inducible system and to methods of use in transgenic animals. More specifically, the present invention relates to a chimeric transcription factor that binds to a ligand and functions in ligand-dependent manner to induce expression of genes of interest under the control of a synthetic operator-promoter sequence. The expression of genes of interest can be tightly controlled by adding or removing the ligand.

LexA-based chemical-inducible gene expression system for use in transgenic animals

A group of genes including GhCIR1 from cotton (), and AtCIR1 and AtCIR2 frompromote shoot regeneration in plants even in the absence of cytokinin. In the presence of cytokinin, the genes significantly improve transformation efficiency. The genes can be used as an enhancer as well as a selectable marker of transformation in plants. The proteins encoded by the novel genes have a homeodomain (HD) at the N-terminus and a highly divergent domain at the C-terminus. The proteins share a common structural motif.

Plant homeodomain protein-encoding genes and their uses

The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

Promotion of somatic embryogenesis in plants by wuschel gene expression

The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing agene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

Chemical inducible promoters used to obtain transgenic plants with a silent marker

A method is provided of regulating transcription in a plant cell from a DNA sequence comprising a target DNA operably linked to a coding sequence, which method comprises introducing an engineered zinc finger polypeptide in said plant cell which polypeptide binds to the target DNA and modulates transcription of the coding sequence.

Regulated gene expression in plants

Growing community of inventors

New York, NY, United States of America

Nam-Hai Chua