Council of Scientific and Industrial Research

Nagesh Peddada

Ashish

Renu Garg

Yogendra Singh Rathore

Kunzes Dolma

The present invention relates to a process which uses blue light to form bio-organic coated silver and gold nanoparticles for various applications. The present invention particularly relates to increasing the rates of reaction and widening the scope of tethering a range of biomolecules on the metal nanoparticles. The examples demonstrate that the biomolecules immobilized on the nanoparticles still retain many aspects of their structural elements which allow them to bind to their native binding partners and/or retain structural epitopes which allow them to be identified by antibodies specific to them, thus opening up scope of using such coated nanoparticles for functionalized and specific applications.

Process for the preparation of bio-organic coated gold and silver nanoparticles using blue light

The present invention relates to novel DNA aptamers capable of binding gelsolin tightly and specifically. The invention further relates to the use of these aptamers to estimate the gelsolin levels in a given sample and purify bulk quantities of tagless gelsolin and its variants. The present invention thus eliminates the use of different animals/their tissues to produce gelsolin binding proteins, which are much more expensive and socially unacceptable methods as opposed to the synthesis of a DNA molecule by in vitro PCR. Using this strategy, bulk production of the gelsolin binding matrix can be carried out at much lower cost. Also, the aptamers can be used to block binding of gelsolin to its binding partners for diagnostic and/or therapeutic applications.

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Chandigarh, India

Nagesh Peddada