New York University

Nadrian C Seeman

James Wayne Canary

Ruojie Sha

Lei Zhu

Paul Michael Chaikin

Xiaoping Zhang

Philip Lukeman

Pamela E Constantinou

Yuwen Zhang

Neville Robert Kallenbach

David Pine

Zhiyong Shen

Yu Liu

Tong Wang

Hong Zhong

Baoquan Ding

Yongli Mi

Jens Kopatsch

Liang Ding

Lisa Israel

William B Sherman

Lisa Wenzler Savin

Furong Liu

Erik Winfree

Jiwen Zheng

Xiaojun Li

Jing Qi

Xiaoping Yang

Xiaojin He

Shiping Liao

Hao Yan

Junghuei Chen

The present invention provides a method for self-replication of multimers of nucleic acid origami tiles by exponentially amplifying the multimer from initial seeds of monomeric units of nucleic acid origami tiles and also provides for the selective exponential amplification of a designated multimer, such as with specific properties or characteristics, over one or more competing multimers in the presence of a mixture of monomers for each of the possible multimers. The selection of the designated multimer based on an environmental change allows the designated multimer to outgrow all competing multimers.

Self-replication of nucleic acid origami tiles

The invention provides micron and sub-micron scale particles designed to recognize and selectively interact with each other by exploiting the recognition and specificity enabled by DNA-sequence-encoded coatings. Such materials possess sufficient information coded in their chemical and physical interactions to self assemble and self replicate. The invention further provides methods of using such materials to create self replicating and organizing materials. Replicated copies are permanently linked and then thermally detached, freeing them to act as templates for further growth. This new class of condensed matter systems, provides means to design and control the structure and function of materials and machines from the microscopic to life-size. In another aspect of the invention, depletion type forces and depletion zones can be utilized in the implementation of the self assembly and self replication of materials, including without limitation colloidal particles. The invention further provides novel means of synthesis and materials built by such synthesis, which may be used in a variety of applications, including microelectronics.

Self-replicating materials

A polynucleic acid nanomechanical device with a linear array of alternating PX-JXdevices and nucleic acid multi-crossover motifs that facilitate the assembly of a nucleic acid strand and functions as an artificial ribosome by translating a nucleic acid signal into an unrelated nucleic acid sequence.

Polynucleotide nanomechanical device that acts as an artificial ribosome and translates DNA signals into polymer assembly instructions

The present invention provides a polyribonucleoside ladder copolymer molecule of general formula (I) 

Nylon polyribonucleosides

The present invention relates to a copolymer termed a ladder copolymer because it has two backbones that serve as legs/sides of a ladder structure. These two backbones, one of which is a nucleic acid or nucleic acid-like polymer, are linked together as the legs/sides of a ladder are linked together by the rungs.

Ladder copolymers

Two dimensional polynucleic acid arrays are assembled from robust nucleic acid motifs as polygonal units. The polygonal units in an array have edges composed of nucleic acid multi-crossover domains and are joined together by double cohesion of adjacent polygonal units. A subset of polygonal units in the array have a nanoparticle or pendant molecule attached to an end of one edge of each polygonal unit within this subset.

Multidimensional organization of heteromolecules by robust DNA motifs

The present invention is directed to a sequence dependent polynucleotide nanomechanical device based on a nucleic acid paranemic crossover (PX) molecule which is a four-stranded structure related to parallel double crossover molecules, except that every possible crossover takes place. Eliminating two crossovers leads to a topoisomer, termed JX, in which one pair of ends are switched (rotated 180&#xb0;) while the other pair of ends remain the same. The device can be cycled between the two states, PX and JX, by replacing single strands that set the state to be PX or JX.

Polynucleic acid nanomechanical device controlled by hybridization topology

Robust nucleic acid arrays and lattices are assembled based on double crossover cohesion of polygonal units whose edges are composed of nucleic acid multi-crossover domains.

Polygonal nanostructures of polynucleic acid multi-crossover molecules and assembly of lattices based on double crossover cohesion

Nucleic acid based ladder copolymers

A multiped, capable of traveling in more than one direction along a molecular path in a nano-robotic system where the steps taken by the feet of the multiped are controlled in a sequence specific fashion, is presented. The feet of the multiped dock to footholds on the molecular path via cohesion with 'set' molecules and are released from the footholds through the introduction of 'unset' molecules that detach or strip away the &#x201c;set&#x201d; molecules.

Nucleic acid based nano-robotic system

Two- and three-dimensional periodic lattices are constructed from repeating units of at least two antiparallel nucleic acid multi-crossover molecules. The repeating units range from single component to multi-component.

Periodic two and three dimensional nucleic acid structures

Two and three dimensional polynucleic acid structures, such as periodic lattices, may be constructed from an ordered array of antiparallel double crossover molecules assembled from single stranded oligonucleotides or polynucleotides. These antiparallel double crossover molecules have the structural rigidity necessary to serve as building block components for two and three dimensional structures having the high translational symmetry associated with crystals.

Nanoconstructions of geometrical objects and lattices from antiparallel

One, two and three dimensional structures may be synthesized or modified from polynucleotides. A core structure is expanded by cleavage of a loop with a restriction endonuclease and ligating another polynucleotide to the sticky ends so that the recognition site of the restriction enzyme is not reformed. This process is repeated as many times as necessary to synthesize any desired structure. The structures formed have a wide range of uses.

Construction of geometrical objects from polynucleotides

The invention discloses an n-connected closed covalent three-dimensional structure of oligonucleotides, n being at least 3, and methods for making and using same.

Growing community of inventors

New York, NY, United States of America

Nadrian C Seeman