Sanofi

Nadja Spindler

Ercole Rao

Christian Lange

Christian Beil

Thomas Langer

Wulf-Dirk Leuschner

Gerhard Hessler

Cendrine Lemoine

Ingo Focken

Katja Kroll

Sandra Weil

Soraya Hoelper

Sevim Ozgur Bruderle

Provided herein are multispecific antibodies, e.g. bispecific antibodies, which are modified such that the desired chain pairing takes place and/or can be selected for. Specifically, this is achieved by using different dimerization domains for light chain pairing. Also disclosed herein are nucleic acids encoding for these antibodies, expression vectors comprising these nucleic acids, cells expressing them, and further to pharmaceutical compositions comprising the antibodies, as well as methods of isolating the antibodies.

Multispecific antibodies facilitating selective light chain pairing

Binding proteins comprising a pseudoFab domain including a stabilised knockout domain and a second VH/VL that form a first functional antigen binding domain are provided. Multispecific binding proteins comprising at least one pseudoFab are also provided. Multispecific binding proteins, nucleic acids encoding binding proteins and multispecific binding proteins, expression vectors, host cells, pharmaceutical composition and methods of treatment administering the binding proteins or multispecific binding proteins described herein are also provided.

PseudoFab-based multispecific binding proteins

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Frankfurt am Main, Germany

Nadja Spindler